T8575
Anti-Tryptophan Hydroxylase Antibody
sheep polyclonal
Synonym(s):
Anti-TPH
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About This Item
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Product Name
Anti-Tryptophan Hydroxylase antibody produced in sheep, affinity isolated antibody, buffered aqueous glycerol solution
biological source
sheep
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
usage
sufficient for 10 blots
species reactivity
mammals, human
technique(s)
western blot: 1:1,000 using human dorsal raphe nucleus
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... TPH1(7166)
mouse ... Tph1(21990)
rat ... Tph1(24848)
General description
The tryptophan hydroxylase 1 (TPH1) gene spanning 29kbp with 11 exons is mapped to human chromosome 11p15.3-p14. TPH1, is a 444 amino acid protein expressed in the gut, spleen, thymus and also in the pineal gland and the pituitary.
Tryptophan Hydroxylase (TPH) is a member of the aromatic amino acid hydroxylases (AAAH) family.
Immunogen
recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
Application
Anti-Tryptophan Hydroxylase antibody produced in sheep has been used in:
- immunocytochemistry
- immunohistochemistry
- immunofluorescence
- immunoperoxidase staining
Biochem/physiol Actions
The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan, which is a precursor to the neurotransmitter serotonin. Variation in the gene expression affects prefrontal cortex activation during response inhibition. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders. The encoded protein plays a vital role in regulation of cardiovascular function.
Physical form
Solution in 150 mM NaCl, 10 mM HEPES, pH 7.5, 100 μg per mL BSA, and 50% glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Matthew W Hale et al.
Journal of chemical neuroanatomy, 41(4), 219-226 (2011-06-07)
Tryptophan hydroxylase is the rate-limiting enzyme in the biosynthesis of serotonin (5-hydroxytryptamine; 5-HT). Two isoforms of tryptophan hydroxylase, derived from different genes, tph1 and tph2, have been identified. The tph1 isoform is expressed in peripheral tissues, whereas tph2 is brain
Tryptophan hydroxylase 1 gene polymorphisms alter prefrontal cortex activation during response inhibition.
Ruocco AC
Neuropsychology, 30, 18-27 (2016)
Rachel A Walker et al.
The European journal of neuroscience, 51(3), 866-880 (2019-08-04)
Aversive, positive prediction error (+PE) provides a mechanism to update and increase future fear to uncertain threat predictors. The ventrolateral periaqueductal grey (vlPAG) has been offered as a neural locus for +PE computation. Yet, a causal demonstration of vlPAG +PE
R C Drugan et al.
Neuroscience, 253, 221-234 (2013-09-04)
Prior adverse experience alters behavioral responses to subsequent stressors. For example, exposure to a brief swim increases immobility in a subsequent swim test 24h later. In order to determine if qualitative differences (e.g. 19°C versus 25°C) in an initial stressor
Matthew W Hale et al.
Experimental neurology, 224(1), 271-281 (2010-04-13)
Serotonin plays an important role in the regulation of anxiety states and physiological responses to aversive stimuli. Intracerebroventricular (i.c.v.) injection of the stress- and anxiety-related neuropeptide urocortin 2 (Ucn 2) increases c-Fos expression in serotonergic neurons in the dorsal (DRD)
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