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SAB4200889

Sigma-Aldrich

Anti-SARS-COV-2-Spike-RBD region Peroxidase conjugated antibody produced in rabbit

Synonym(s):

ACE2-binding protein, Angiotensin Converting Enzyme 2 (ACE2)-binding region, COVID-19 Spike protein, Coronavirus, RBD Region, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2), Spike surface glycoprotein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

form

lyophilized powder

Quality Level

packaging

vial of 100 μL

concentration

3-5 mg/mL

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) or (2019-nCoV) is a novel coronavirus that had spread on December 2019 in Hubei province of China and infected millions of people worldwide.1 The causative agent of COVID-19, the SARS-CoV-2 virus is a positive-strand RNA virus .The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S), E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The main receptor for SARS-CoV and SARS-CoV-2 on the membrane of the target cells is the Angiotensin 2 Converting Enzyme (ACE2). ACE2 is a metallopeptidase present on the membrane of many cells, including type-I and -II pneumocytes, small intestine enterocytes, kidney proximal tubules cells, the endothelial cells of arteries and veins, and the arterial smooth muscle, among other tissues.15-16 It has been shown that SARS-CoV-2 virus employs transmembrane protease serine 2 (TMPRSS2) for S protein priming and it is speculated that furin-mediated cleavage at the S1/S2 site in infected cells, may promote subsequent TMPRSS2-dependent entry into target cells.

Specificity

Anti-SARS-CoV-2-Spike-RBD region antibody specifically recognizes Spike from COVID-19 virus origin.

Application

The antibody may be used in various immunochemical techniques including Immunoblotting and Elisa. Detection of the Spike RBD protein band by Immunoblotting is specifically inhibited by the immunogen.

Biochem/physiol Actions

The Spike protein (S) is responsible for virus binding and entry into the host cells. SARS-CoV-2 S protein precursor is cleaved into S1 subunit (685 amino acids), and S2 (588 amino acids) subunits. S1 subunit harbor the receptor binding domain (RBD) that mediates virus entry into susceptible cells through the peptidase domain of host ACE2 with high affinity (Kd = 15 nM). S2 protein, which is reported to be well-conserved and showing 99% identity with bat coronavirus, is responsible for the membrane fusion. The Spike protein is the most studied between the coronaviruses proteins, due to its crucial role in the host cell entry, it contains the RBD for the ligand on the host cell membrane (the ACE2 protein), and also has epitopes recognized by T and B cells, which induce the production of neutralizing antibodies.2

Storage and Stability

Store the lyophilized product at 2–8 °C. For extended storage after reconstitution, keep at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 °CC for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Data presented is the available current product information and provided as-is. This product has not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2


Certificates of Analysis (COA)

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Furong Qi et al.
Biochemical and biophysical research communications, 526(1), 135-140 (2020-03-23)
The new coronavirus (SARS-CoV-2) outbreak from December 2019 in Wuhan, Hubei, China, has been declared a global public health emergency. Angiotensin I converting enzyme 2 (ACE2), is the host receptor by SARS-CoV-2 to infect human cells. Although ACE2 is reported
[Iatrogenic bilateral pneumothorax after tracheostomy in childhood].
V A Kharitonov et al.
Pediatriia, (7), 88-88 (1988-01-01)
Kento T Abe et al.
JCI insight, 5(19) (2020-09-02)
Most of the patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mount a humoral immune response to the virus within a few weeks of infection, but the duration of this response and how it correlates with clinical outcomes
Jun Lan et al.
Nature, 581(7807), 215-220 (2020-04-01)
A new and highly pathogenic coronavirus (severe acute respiratory syndrome coronavirus-2, SARS-CoV-2) caused an outbreak in Wuhan city, Hubei province, China, starting from December 2019 that quickly spread nationwide and to other countries around the world1-3. Here, to better understand the
Zhixin Liu et al.
Journal of medical virology, 92(6), 595-601 (2020-02-27)
From the beginning of 2002 and 2012, severe respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) crossed the species barriers to infect humans, causing thousands of infections and hundreds of deaths, respectively. Currently, a novel coronavirus (SARS-CoV-2)

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