One nanomole of substrate or less can be cleaved in a 20 μL reaction volume. In the standard reaction use: 4 μL of 5X enzyme reaction "buffer B" (=250 mM sodium phosphate buffer, pH 6.0), a combined volume of 14 μL containing purified oligosaccharide and water and 2 μL of NANase I (N7271). Incubate 1 hour at 37 °C. To cleave more than one nanomole of substrate, increase reaction volume and enzyme proportionally.
N7271
α(2→3) Neuraminidase from Streptococcus pneumoniae
buffered aqueous solution
Synonym(s):
Acyl-neuraminyl Hydrolase, Acylneuraminyl hydrolase, Receptor-destroying enzyme, Sialidase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Biological source:
Streptococcus pneumoniae
Concentration:
≥5 units/mL
Pricing and availability is not currently available.
biological source
Streptococcus pneumoniae
Quality Level
form
buffered aqueous solution
concentration
≥5 units/mL
foreign activity
β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected
shipped in
wet ice
storage temp.
2-8°C
Gene Information
Streptococcus pneumoniae R6 ... nanB(933504)
Biochem/physiol Actions
Releases α(2→3)-linked N-acetylneuraminic acid from complex oligosaccharides.
Packaging
Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).
Physical form
Solution in 50 mM sodium phosphate, pH 7.5
Preparation Note
Expressed in glycosidase-free hosts.
Other Notes
One unit will hydrolyze 1μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| Gene Information Streptococcus pneumoniae R6 ... nanB(933504) | Gene Information - | Gene Information Clostridium perfringens str. 13 ... nanI(988807) | Gene Information Clostridium perfringens str. 13 ... nanI(988807) |
| biological source Streptococcus pneumoniae | biological source - | biological source - | biological source - |
| form buffered aqueous solution | form buffered aqueous solution | form lyophilized powder | form lyophilized powder |
| concentration ≥5 units/mL | concentration - | concentration - | concentration - |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Datasheet
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| N7271-.2UN | 04061832856773 |
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What are the reaction conditions of the enzyme ?(2—›3) Neuraminidase, Product No. N7271?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of ?(2—›3) Neuraminidase, Product No. N7271?
1 answer-
The molecular weight of this ?(2—›3 Neuraminidase enzyme is approximately 75 kDa.
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What is the long term storage and shipping condition of ?(2—›3) Neuraminidase, Product No. N7271?
1 answer-
It is shipped at ambient temperature, but it is to be stored at 2-8 °C for long term storage. Do not FREEZE as it is in a buffered aqueous solution.
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For ?(2—›3) Neuraminidase, Product No. N7271, what is the optimum pH range for the neuraminidase enzyme activity?
1 answer-
The optimum pH range for ?(2—›3 neuraminidase is 6.0. Please see the data sheet.
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