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4-Methylumbelliferyl β-D-glucopyranoside

Name: 4-Methylumbelliferyl &#946;-<SC>D</SC>-glucopyranoside
Brand: SIGMA

β-glucosidase substrate, fluorogenic, ≥99% (HPLC), powder

Synonym(s):

MUD

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About This Item

Empirical Formula (Hill Notation):

C₁₆H₁₈O₈

CAS Number:

18997-57-4

Molecular Weight:

338.31

NACRES:

NA.32

PubChem Substance ID:

24896859

UNSPSC Code:

12352204

EC Number:

242-736-7

MDL number:

MFCD00063694

Beilstein/REAXYS Number:

94672

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Properties

Product Name

4-Methylumbelliferyl β-D-glucopyranoside, β-glucosidase substrate

Quality Segment

200

assay

≥99% (HPLC)

form

powder

solubility

DMF: 50 mg/mL, clear, colorless to faintly yellow

fluorescence

λ_ex 316 nm; λ_em 372 nm (pH 9.1), λ_ex 360 nm; λ_em 449 nm (Reaction product)

storage temp.

−20°C

SMILES string

CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13-,14+,15-,16-/m1/s1

InChI key

YUDPTGPSBJVHCN-YMILTQATSA-N

Description

Application

4-Methylumbelliferyl β-D-glucopyranoside has been used as substrate:

in glucosylceramidase β enzyme activity assay in lysosome-enriched fractions from primary hippocampal neurons
in β-glucosidase assay during yeast fermentation
to assay glucocerebrosidase 1 (GBA1)-related glucosidase activity in macrophage cell line (RAW)

Biochem/physiol Actions

4-Methylumbelliferyl β-D-glucopyranoside is a synthetic enzymatic substrate for glycosidase. It has been used as substrate for the β- glucosidase from enterococci.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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This Item	M7633	M9766	M9659
Sigma-Aldrich M3633 4-Methylumbelliferyl β-D-glucopyranoside	Sigma-Aldrich M7633 4-Methylumbelliferyl α-D-galactopyranoside	Sigma-Aldrich M9766 4-Methylumbelliferyl α-D-glucopyranoside	Sigma-Aldrich M9659 4-Methylumbelliferyl N-acetyl-β-D-galactosaminide
assay ≥99% (HPLC)	assay ≥98% (TLC)	assay ≥99% (TLC)	assay ≥98% (HPLC)
solubility DMF: 50 mg/mL, clear, colorless to faintly yellow	solubility water: 50 mg/mL, clear, colorless to very faintly yellow	solubility pyridine: 50 mg/mL, clear, colorless to faintly yellow	solubility pyridine: 50 mg/mL, clear, colorless to faintly yellow
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 200
form powder	form powder	form powder	form powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
fluorescence λ_ex 316 nm; λ_em 372 nm (pH 9.1), λ_ex 360 nm; λ_em 449 nm (Reaction product)	fluorescence λ_ex 317 nm; λ_em 374 nm (pH 9.1), λ_ex 360 nm; λ_em 449 nm (Reaction product)	fluorescence -	fluorescence -

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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