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I2911

Sigma-Aldrich

IMDM

StableCell™, with stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Iscove’s Modified Dulbecco’s Medium, IMDM

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About This Item

MDL number:
MFCD00217469
UNSPSC Code:
12352207
NACRES:
NA.75

product name

StableCell™ IMDM, With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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General description

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Legal Information

StableCell is a trademark of Sigma-Aldrich Co. LLC

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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