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G9785

Sigma-Aldrich

GSEM Supplement

liquid, suitable for cell culture,

Synonym(s):

Glutamine synthetase expression medium supplement

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About This Item

MDL number:
MFCD00677096
UNSPSC Code:
12352205
NACRES:
NA.75

product name

GSEM Supplement, 50 ×, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

concentration

50 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

shipped in

dry ice

storage temp.

−20°C

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General description

Glutamine synthetase expression medium (GSEM) supplement enables formation of glutamine from ammonia and glutamate there by alleviating accumulation of ammonia in culture medium. It is exploited for high yield recombinant protein production in Chinese hamster ovary (CHO) cells.

Application

GSEM Supplement has been used as a supplement for ProCHO5 Protein-free medium for culturing Chinese hamster ovary (CHO) cells and selective medium for NS0 myeloma cells.

Packaging

Contains (mg/L): L-alanine 450.0, L-asparagine · H2O 4261.0, L-aspartic acid 650.0, L-glutamic acid 3750.0, L-proline 575.0, L-serine 500.0, adenosine 350.0, guanosine 350.0, cytidine 350.0, uridine 350.0, and thymidine 12.0

Quantity

One mL will supplement 50 mL of cell culture medium.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Comprehensive characterization of glutamine synthetase-mediated selection for the establishment of recombinant CHO cells producing monoclonal antibodies
Noh SM, et al.
Scientific reports, 8(1), 5361-5361 (2018)
Soluble Fc fusion proteins for biomedical research
Monoclonal Antibodies: Methods and Protocols, 33-52 (2007)
Novel membrane?bound reporter molecule for sorting high producer cells by flow cytometry.
Helman D, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 85(2), 162-168 (2014)
Doyle, A., et al.
Cell and Tissue Culture: Laboratory Procedures, 27D, 3-3 (1999)
A streamlined implementation of the glutamine synthetase-based protein expression system
Knox R, et al.
BMC biotechnology, 13(1), 74-74 (2013)
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