No, this product is not endotoxin-free. Endotoxin levels are determined on a lot-to-lot basis with the maximum allowable level of 10.0 EU/mL. Please see the link below to review the specifications of this product:
https://www.sigmaaldrich.com/documents/spec/F9665/SIGMA
F9665
Fetal Bovine Serum
Heat Inactivated, non-USA origin, sterile-filtered, suitable for cell culture
1 of 2 reviewers received a sample product or took part in a promotion
Synonym(s):
HI FBS, HI FCS, HI Fetal Calf Serum, HI Serum, HI sera, Heat Inactivated Fetal Calf Serum, FBS, FCS, sera, serum
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About This Item
Biological source:
bovine fetus
Origin:
non-USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
Pricing and availability is not currently available.
biological source
bovine fetus
Quality Segment
sterility
sterile-filtered
product line
FBS Classic
composition
Hemoglobin, ≤25 mg/dL
origin
non-USA origin
availability
not available in USA
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
Application
FBS is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Preparation Note
Prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes is used to inactivate the complement system for immunoassays. Heat inactivation also inactivates other undetermined inhibitors of cell growth in culture.
Analysis Note
- Endotoxin and hemoglobin tested
- Tested for the presence of bacteria, virus, and mycoplasma
- Triple filtered with 0.1 micron membrane under aseptic conditions
Legal Information
Not available in USA or Canada. Please check with your local supplier regarding availability and import restrictions in your country.
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This Item | |||
|---|---|---|---|
| description Heat Inactivated, non-USA origin, sterile-filtered, suitable for cell culture | description USA origin, γ-irradiated by SER-TAIN process, sterile-filtered, suitable for cell culture | description USA origin, Dialyzed by ultrafiltration against 0.15 M NaCl, sterile-filtered, suitable for cell culture | description Canada origin, sterile-filtered, suitable for cell culture |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| origin non-USA origin | origin USA origin | origin USA origin | origin Canada origin |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin |
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Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves
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Is this FBS endotoxin free?
1 answer-
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Do you know if this FBS is antibiotic or tetracycline free?
1 answer-
This serum has not been tested for the presence of antibiotics or tetracycline.
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Une fois ouverte combien de temps le produit peut être utilisé?
1 answer-
After thawing, when stored refrigerated, fetal bovine serum is stable for approximately 1-2 weeks for use in cell culture. Serum intended for use with more robust cell lines, in analytical assays, or for blocking applications, may be stable up to 30 days when stored refrigerated and assuming no contamination has occurred.
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Do you maybe know the range of zinc concentration in your FBSin?
1 answer-
Unfortunately, the zinc concentration for this product is not determined.
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It stated 'heat-inactivated' should one perform inactivation upon product arrival?
1 answer-
Heat inactivation is not necessary. This Fetal Bovine Serum is already heat inactivated.
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