The amplified DNA should be loaded onto an agarose gel after the PCR is completed by adding a separate loading buffer or tracking dye, such as Gel Loading Solution, Catalog Number G2526.
E3004
Extract-N-Amp™ PCR ReadyMix™
Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue
Synonym(s):
Plant direct PCR master mix, Tissue direct PCR master mix
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About This Item
NACRES:
NA.55
UNSPSC Code:
41106303
Pricing and availability is not currently available.
Quality Segment
usage
sufficient for 100 reactions, 1.2 mL sufficient for 100 amplifications, sufficient for 1000 reactions, 12 mL sufficient for 1000 amplifications, sufficient for 10000 reactions, 125 mL sufficient for 10000 amplifications
feature
dNTPs included, hotstart, Direct PCR
technique(s)
PCR: suitable, whole genome amplification: suitable
color
colorless
input
crude DNA
application(s)
agriculture
cannabis testing
shipped in
wet ice
storage temp.
−20°C
General description
Extract-N-Amp™ PCR ReadyMix™ is a specially formulated hot start PCR master mix for direct PCR amplification from the extract.
PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits include a PCR ReadyMix sufficient for one PCR reaction per extraction. However, if additional PCR reactions are required, supplemental PCR ReadyMix may be needed.
Application
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
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| description Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue | description 125 mL sufficient for 10000 amplifications, hotstart, dNTPs included | description sufficient for 1000 extractions, sufficient for 1000 amplifications | description Ready-to-use 2X PCR Master Mix with Loading Dye |
| technique(s) whole genome amplification: suitable, PCR: suitable | technique(s) - | technique(s) PCR: suitable, whole genome amplification: suitable | technique(s) whole genome amplification: suitable |
| input crude DNA | input - | input crude DNA | input - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| color colorless | color - | color colorless | color red |
| usage 1.2 mL sufficient for 100 amplifications, 12 mL sufficient for 1000 amplifications, sufficient for 1000 reactions, sufficient for 10000 reactions, 125 mL sufficient for 10000 amplifications, sufficient for 100 reactions | usage 125 mL sufficient for 10000 amplifications, sufficient for 10000 reactions | usage sufficient for 1000 amplifications, sufficient for 1000 extractions | usage 125 mL sufficient for 10000 amplifications, sufficient for 100 reactions, 1.2 mL sufficient for 100 amplifications, sufficient for 10000 reactions, sufficient for 1000 reactions, 12 mL sufficient for 1000 amplifications |
Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Sehr geehrte Damen und Herren, sollen wir nach dem PCR Lauf, Ladepuffer dazugeben?
1 answer-
Helpful?
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Can I use the TriTrack DNA Loading Dye or the TrakitCyanoOrange Loading Buffer with the ReadyMix??
1 answer-
The choice of loading dye will not impact the performance the the E3004 - Extract-N-Amp™ PCR ReadyMix. While we have not tested loading dyes outside of the product, G2526, any commercial DNA loading dye is expected to be compatible.
Helpful?
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What is the polymerase used for this kit? I am trying to calculate Tm for my PCR, but I would need to know what type of polymerase this kit uses. I am guessing something close to Q5 Hotstart?
1 answer-
This kit employs Taq DNA polymerase (Product D6677).
Helpful?
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