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D7777

Dulbecco′s Modified Eagle′s Medium - high glucose

With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

DME, DMEM

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207
Pricing and availability is not currently available.
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Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: high
sodium pyruvate: yes
NaHCO3: no
HEPES: no
phenol red: yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Preparation Note

Formulated to contain 13.5 grams of powder per liter of medium.
Supplement with 3.7 g/L sodium bicarbonate

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D1152D5648D2902
Sigma-Aldrich

Sigma-Aldrich

D7777

Dulbecco′s Modified Eagle′s Medium - high glucose

Sigma-Aldrich

Sigma-Aldrich

D1152

Dulbecco′s Modified Eagle′s Medium - high glucose

Dulbecco’s Modified Eagle’s Medium - high glucose With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

D5648

Dulbecco′s Modified Eagle′s Medium - high glucose

Sigma-Aldrich

Sigma-Aldrich

D2902

Dulbecco′s Modified Eagle′s Medium - low glucose

form

powder

form

powder

form

powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

glucose: high
sodium pyruvate: yes
NaHCO3: no
HEPES: no
phenol red: yes
L-glutamine: yes

components

sodium pyruvate: no
NaHCO3: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high

components

phenol red: yes
L-glutamine: yes
NaHCO3: no
HEPES: no
glucose: high
sodium pyruvate: no

components

glucose: low
NaHCO3: no
phenol red: no
HEPES: no
L-glutamine: yes
sodium pyruvate: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

500

Quality Level

-

Quality Level

500

Quality Level

-

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Storage Class

13 - Non Combustible Solids

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Certificates of Analysis (COA)

Lot/Batch Number
0000537817
0000534920
0000518984
0000518982
0000518981

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Global Trade Item Number

SKUGTIN
D7777-25KGS04065266904253
D7777-BIBC04061826573624
D7777-10X1L04061833588864
D7777-50L04061833588871
D7777-10L04061833588857

Questions

1–2 of 2 Questions  

  1. What format does the 10x1L powder come in? Is it glass bottles or pouches?

    1 answer

    1. Each 1 L quantity is packaged in a 30 mL amber glass bottle with 33-400 threading and a red polypropylene cap.

      Helpful?

  2. Hello, can you provide me the exact concentration of L-glutamine and sodium pyruvate present in this DMEM medium.

    1 answer

    1. The formulation is available on page 3 under DOCUMENTATION or at this link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/947/141/d7777for.pdf

      Helpful?

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