D5652

Dulbecco′s Phosphate Buffered Saline

Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture

• Synonym(s): DPBS
• UNSPSC Code: 12352207
• eCl@ss: 32129211
• NACRES: NA.25
• MDL number: MFCD00131855

Properties

• Quality Segment: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• pH: 7.2-7.8
• solubility: water: 9.6 g/L, clear
• storage temp.: 2-8°C

Description

General description

Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture.

Application

D-PBS is used to wash cells during preparation and serial transfer.

Dulbecco′s Phosphate Buffered Saline has been used:

• as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloy
• to dissolve Angelica gigas powder for microencapsulation
• to wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assay
• to wash amniotic membrane for cellular analyses

Biochem/physiol Actions

Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.

Preparation Note

Formulated to contain 9.6 grams of powder per liter of medium.

Other Notes

This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable