The solubility and stability of this product when reconstituted in PBS has not been evaluated. The DAB tablets do include a Tris buffer and the Urea tablets include phosphate buffer salts. Reconstitution in water is recommended for both components. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/815/398/d4168dat-ms.pdf
D4168
SIGMAFAST™ 3,3′-Diaminobenzidine tablets
SIGMAFAST™, tablet
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About This Item
CAS Number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:
Pricing and availability is not currently available.
Product Name
SIGMAFAST™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 1 mL
Quality Segment
form
tablet
technique(s)
protein detection: suitable
solubility
deionized water: 1 mL, clear, pink
storage temp.
−20°C
SMILES string
Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI
1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2
InChI key
HSTOKWSFWGCZMH-UHFFFAOYSA-N
Application
Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets
Preparation Note
Each tablet set dissolved in 1 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.
Legal Information
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| description tablet, To prepare 1 mL | description tablet, To prepare 5 mL | description tablet, To prepare 15 mL | description 97% (HPLC) |
| solubility deionized water: 1 mL, clear, pink | solubility - | solubility water: 1 tablet/5 mL | solubility - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form tablet | form tablet | form tablet | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. room temp |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Ox. Liq. 3 - Skin Irrit. 2 - STOT SE 3
Target Organs
Central nervous system
Storage Class
5.1B - Oxidizing hazardous materials
Flash Point (°F)
53.6 °F - closed cup
Flash Point (°C)
12 °C - closed cup
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Can it be dissolved in PB? Thank you.
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