Black plates with clear, flat bottoms are recommended for bottom reading instruments, while solid black plates are recommended for top reading instruments.
CS0005
Cholesterol Quantification Assay kit
Synonym(s):
Cholesterol Assay kit, cholesteryl ester, free cholesterol, total cholesterol
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About This Item
NACRES:
NA.83
UNSPSC Code:
12352202
Pricing and availability is not currently available.
usage
sufficient for 100 or 400 colorimetric or fluorometric tests
shipped in
dry ice
storage temp.
−20°C
General description
Cholesterol is a lipid that constitutes 20-25% of the structural components of cell membranes. Cholesterol presence assure membrane structural fluidity. Cholesterol also plays a role as an intermediate in several hormones and bile acid biosynthesis and vitamin D. Cholesterol also regulates the functions of the transporters and signaling proteins present on the plasma membrane. The major sites of cholesterol synthesis are the small intestine and liver. Cholesterol circulates in the blood as both the free acid and as cholesterol esters. Increased cholesterol levels are associated with the development of atherosclerosis and cardiovascular diseases. Thus, serum cholesterol regulation has an important therapeutic role.
The Cholesterol Quantification Assay Kit provides a simple and quick procedure for detecting and measuring very low concentrations of Cholesterol. This kit can measure free cholesterol, cholesteryl esters, or total cholesterol (which constitutes both free cholesterol and cholesteryl esters). The cholesterol concentration is determined via a coupled enzymatic reaction. This kit allows for either fluorometric or colorimetric detection, to allow maximum flexibility and a large detection range. The linear ranges are as follows:
The Cholesterol Quantification Assay Kit provides a simple and quick procedure for detecting and measuring very low concentrations of Cholesterol. This kit can measure free cholesterol, cholesteryl esters, or total cholesterol (which constitutes both free cholesterol and cholesteryl esters). The cholesterol concentration is determined via a coupled enzymatic reaction. This kit allows for either fluorometric or colorimetric detection, to allow maximum flexibility and a large detection range. The linear ranges are as follows:
- Fluorescence assay: 0.1–0.5 μg
- Colorimetric assay: 1–5 μg
Application
- Measuring free cholesterol, cholesteryl esters, or total cholesterol in biological samples (For example: Serum and Plasma and tissue culture).
- Measurement can be done either by fluorometric or colorimetric detection.
Suitable for the detection of free cholesterol, cholesteryl esters, or total cholesterol in biological samples.
Features and Benefits
- Highly sensitive, simple and high-throughput assay for measuring free cholesterol, cholesteryl esters, or total cholesterol in biological samples
- User friendly: No need to weigh or calculate needed amounts of reagents on your own
- Supportive calculator (Click here to download a calculator excel file): Will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data!
- Quick instruction bench card - to assure your experimental success
- Suitable for both colorimetric and fluorometric detection
- More experiments in one kit - contains sufficient reagents for 100 colorimetric or 400 fluorometric tests.
- Detection time: only 30 minutes
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This Item | |||
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| usage sufficient for 100 or 400 colorimetric or fluorometric tests | usage - | usage - | usage sufficient for 100 fluorometric tests |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in - |
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Storage Class
10 - Combustible liquids
wgk
WGK 3
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You are advising to use black plates for fluorescence assays. Are you talking about black plates with black bottom or black plates with optical bottom (see-through) ?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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