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HeLa Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93021013, human cervix, Epithelial

Synonym(s):

HeLa Cells, HeLa-CCL2 Cells, Henrietta Lacks Cells

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About This Item

UNSPSC Code:
41106514
Biological source:
human cervix
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
aneuploidy
Morphology:
Epithelial
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biological source

human cervix

packaging

tube of 5 μg 93021013-DNA-5UG, pkg of vial of cells 93021013-1VL

growth mode

Adherent

karyotype

aneuploidy

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Virus studies
HeLa cell line has been used to study whether Zika virus (ZIKV) infection alters centrosome number and spindle positioning in neural progenitor cells. It has also been used to evaluate the cytotoxicity of extracts, fruits and jams from Sorbus sps.

Biochem/physiol Actions

Derived from a cervical carcinoma from a 31 year old female. This was the first aneuploid line derived from human tissue maintained in continuous cell culture. Susceptible to Poliovirus type I and adenovirus type 3. Identified as a contaminant in many other cell lines. The cells should be handled under laboratory containment level 2. Ethnicity: Black.
Human cervix epitheloid carcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 12,14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino AcidsNEAA + 10% Foetal Bovine Serum FBS / FCS.
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1.3x10,000 cells/cm2 using 0.25% Trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].

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Questions

1–9 of 9 Questions  

  1. Bonjour, y a-t-il un pré-requis de Biosafety Level pour pouvoir commander ces cellules? Merci

    1 answer

    1. As indicated in the 'DESCRIPTION' section of the product page, under 'Cell Line Description', these cells should be handled under laboratory containment level 2.

      Helpful?

  2. Hey! Regarding 93021013-DNA-5UG: Is this the cell line (frozen culture) or really (isolated?) DNA? Thanks, hannes

    1 answer

    1. 93021013-DNA-5UG is DNA extracted from the cell line.

      Helpful?

  3. Are the Cell Line cells tested for viral pathogens?

    1 answer

    1. HPA Cultures does not perform any viral testing on the cell lines.

      Helpful?

  4. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    1 answer

    1. If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

      Helpful?

  5. How do I handle Cell Line frozen cells upon arrival?

    1 answer

    1. Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

      Helpful?

  6. What medium should I use when I thaw the Cell Line cells?

    1 answer

    1. The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

      Helpful?

  7. How many Cell Line cells are in the vial?

    1 answer

    1. Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

      Helpful?

  8. Are my Cell Line cells mycoplasma tested?

    1 answer

    1. ECACC routinely tests all manufactured cell banks for mycoplasma.

      Helpful?

  9. What passage are my Cell Line cells?

    1 answer

    1. If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

      Helpful?

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