C1821
Monoclonal Anti-Pan Cadherin antibody produced in mouse
clone CH-19, ascites fluid
Synonym(s):
Monoclonal Anti-Pan Cadherin
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
CH-19, monoclonal
mol wt
antigen 135 kDa
contains
15 mM sodium azide
species reactivity
canine, frog, snake, guinea pig, chicken, pig, feline, bovine, rabbit, hamster, Psammomys (sand rat), goat, rat, human, sheep, mouse
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested animal heart sections
indirect immunofluorescence: 1:500 using cultured MDBK cells
microarray: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CDH1(999) , CDH2(1000) , CDH3(1001)
mouse ... Cdh1(12550) , Cdh2(12558) , Cdh3(12560)
rat ... Cdh1(83502) , Cdh2(83501) , Cdh3(116777)
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General description
Cadherins are members of a multigene family of single chain glycoprotein receptors mediating Ca2+ -dependent cell-cell adhesion. The N-terminal part of these molecule is exposed on the external cell surface and contains the putative homophilic binding sites. This is followed by a typical single transmembrane sequence and usually a cytoplasmic C-terminal tail which mediates interaction with the microfilament system through molecules such as catenins, plakoglobin, vinculin, and α-actinin.
Cadherins that are primarily located in areas of cell-cell contacts are involved in selective cell sorting and in the mechanical cytoplasmic response. They are implicated in morphogenetic processes, intercellular signalling and in tumor invasiveness and metastasis.
Multiple cadherins have been characterized from diverse species and tissues including E-Cadherin, N-Cadherin (A-CAM), P-Cadherin, V-Cadherin, R-Cadherin and T-Cadherin. Specific polyclonal antibodies against a highly conserved sequence from the cytoplasmic C-terminal of N-Cadherin has been prepared.
Cadherins that are primarily located in areas of cell-cell contacts are involved in selective cell sorting and in the mechanical cytoplasmic response. They are implicated in morphogenetic processes, intercellular signalling and in tumor invasiveness and metastasis.
Multiple cadherins have been characterized from diverse species and tissues including E-Cadherin, N-Cadherin (A-CAM), P-Cadherin, V-Cadherin, R-Cadherin and T-Cadherin. Specific polyclonal antibodies against a highly conserved sequence from the cytoplasmic C-terminal of N-Cadherin has been prepared.
Neural-cadherin (N-cadherin) is a member of the classic cadherin family. It is mainly expressed in neural and mesenchymal tissues.
Specificity
Detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, stains adherens type cell-cell junctions regardless of their cadherin type.
Monoclonal Anti-Pan Cadherin antibody reacts extensively with all known members of the cadherin family. The cadherins migrate in the molecular weight range of 125 to 140 kDa and are tissue dependent. The antibody reacts using frozen sections, protease-digested, formalin-fixed paraffin-embedded, Bouin′s-fixed and acetone-fixed tissue sections. It reacts with cultured cell line preparations. Cross-reaction has been observed with human, rabbit, bovine, goat, sheep, dog, pig, guinea pig, hamster, cat, rat, mouse, psammomys, chicken, frog and snake.
Immunogen
synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled to KLH.
Application
Monoclonal Anti-Pan Cadherin antibody produced in mouse has been used in:
- fluorescence imaging
- immunohistochemistry
- western blotting
- immunoblotting
- immunofluorescence
Monoclonal Anti-Pan Cadherin may be used for: immunochemical and immunocytochemical detection of members of the cadherin family in normal and neoplastic cells and tissues; identification of genetically engineered proteins containing the C-terminal cadherin tail; screening of cDNA expression libraries for identification of novel members of the cadherin family and demonstration of adherens-type cell-cell junctions irrespective of their cadherin subtype.
Biochem/physiol Actions
Neural-cadherin (N-cadherin) has an oncogenic role in bladder cancer, breast cancer, prostate cancer and melanoma. It stimulates the heterotypic adhesion of melanoma cells to dermal fibroblast cells. N-cadherin acts as a critical modulator for the migration of neuronal progenitors. It is essential for the proper cohesion and polarisation of neuroepithelial cells. N-cadherin is important for normal gastrulation and neural crest development. It is required for myogenesis and myotube formation.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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