B4554

BES

BioPerformance Certified, suitable for cell culture, ≥99.0%

• Synonym(s): N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine
• Empirical Formula (Hill Notation): C₆H₁₅NO₅S
• CAS Number: 10191-18-1
• Molecular Weight: 213.25
• Beilstein: 1781572
• EC Number: 233-465-5
• MDL number: MFCD00007533
• UNSPSC Code: 12161700
• PubChem Substance ID: 24891788
• NACRES: NA.25

Properties

• grade: BioPerformance Certified
• Assay: ≥99.0%
• form: crystalline
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin and bioburden, tested
• pH: 2.5-5.0 (25 °C, 213.3 g/L)
• useful pH range: 6.4-7.8
• pKa (25 °C): 7.1
• solubility: water: 0.5 g/mL, clear, colorless
• application(s): diagnostic assay manufacturing
• foreign activity: DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
• storage temp.: room temp
• SMILES string: OCCN(CCO)CCS(O)(=O)=O
• InChI: 1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
• InChI key: AJTVSSFTXWNIRG-UHFFFAOYSA-N

Description

General description

N-N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES) is a zwitterionic sodium sulphate buffer used in biochemistry and molecular biology research. BES buffer used as a secondary pH standard can help maintain a stable pH range in solutions, particularly in the range of 6.2-7.6 pH range of 6.6 to 7.4. It ​is used in a study to evaluate that buffers minimize the action of interfering factors in the bacterial endotoxins test. The application of BES as a buffer solution for calcium phosphate-mediated transfection of eukaryotic cells with plasmid DNA has also been studied.

Application

BES buffer has been used:

• as a supplement in opti-minimal essential medium (MEM) culture media to culture thymocytes for In vitro analysis of thymocyte apoptosis
• as a component of borate buffer saline (BBS) for adeno-associated virus (AAV) vector production, extraction, and purification
• as a component of diluent one for semen collection and cryopreservation

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves