The activity and protein concentration of this product varies from lot to lot and are reported in the product Certificate of Analysis. This information may then be used to determine the lot specific volume. As an example, a lot reporting a 99 mg/mL protein concentration would result in a volume of 2.53 mL for the 250 mg package size. This 250 mg package would have a total activity of 2436 units. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/76427#product-documentation
76427
Penicillin Amidase from Escherichia coli
5-10 units/mg protein
Synonym(s):
Penicillin Acylase, Penicillin Amidohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-753-8
MDL number:
Specific activity:
5-10 units/mg protein
Biological source:
Escherichia coli
Pricing and availability is not currently available.
biological source
Escherichia coli
Quality Segment
form
suspension
specific activity
5-10 units/mg protein
mol wt
Mr ~70000
technique(s)
activity assay: suitable
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
General description
Penicillin amidase is a periplasmic 80K heterodimer with A and B chains (209 and 566 amino acids, respectively). It is widely distributed among microorganisms, including bacteria, yeast and filamentous fungi. Among all the sources, the enzyme produced by E. coli is most well-characterized and common for industrial application.[1]
Application
Penicillin amidase was used to study its effect in release of fatty acid and HSL (homoserine lactone) from AHLs (N -acylhomoserine lactones) in degradation of antibiotics.[2] It was used as positive control for assaying penicillin G acylase activity in the study of functional analysis of bile salt hydrolase and penicillin acylase family members in Lactobacillus sp.[3] Penicillin amidase may be used for synthesis of 6-aminopenicillanic acid from penicillin-G and for the industrial production of β-lactam antibiotics.
Biochem/physiol Actions
The biosynthesis of Penicillin amidase in E. coli by hydrophobic protein chromatography is an inducible reaction which is regulated by metabolized carbon source (e.g. polyols, carboxylic acid etc.). It is also influenced by catabolite repression.[4] It catalyzes the formation of amide bonds through an acyl-enzyme intermediate.
Other Notes
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol benzylpenicillin per minute at pH 7.6 and 37°C
Characterization; In enantioselective resolution; Synthesis of ampicillin and benzylpenicillin.
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This Item | |||
|---|---|---|---|
| specific activity 5-10 units/mg protein | specific activity ≥50 units/mg protein | specific activity 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer) | specific activity 100-300 units/mg protein (biuret) |
| technique(s) activity assay: suitable | technique(s) - | technique(s) - | technique(s) - |
| biological source Escherichia coli | biological source Escherichia coli | biological source Escherichia coli | biological source - |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) - |
| form suspension | form lyophilized powder | form ammonium sulfate suspension | form lyophilized powder |
| mol wt Mr ~70000 | mol wt 110 kDa | mol wt - | mol wt - |
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Good afternoon, I recently received Penicillin-Amidase aus E. coli 5-10 units/mg protein (250mg). Could you please guide me how much volume does it contain so that we can calculate the precise amount of concenteration available. Thank you in advance.
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