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Osmium tetroxide solution

Name: Osmium tetroxide solution
Brand: SIGMA

suitable for electron microscopy, 2% in H₂O

Synonym(s):

Osmium(IV) oxide

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About This Item

Linear Formula:

OsO₄

CAS Number:

20816-12-0

Molecular Weight:

254.23

NACRES:

NA.47

PubChem Substance ID:

57652577

UNSPSC Code:

12171500

MDL number:

MFCD00011150

Form:

solution

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Properties

form

solution

Quality Segment

100

reaction suitability

reagent type: oxidant

concentration

2% in H₂O

technique(s)

electron microscopy: suitable

color

colorless to yellow

density

1.0 g/mL at 20 °C

application(s)

hematology
histology

storage temp.

2-8°C

SMILES string

O=[Os](=O)(=O)=O

InChI

1S/4O.Os

InChI key

VUVGYHUDAICLFK-UHFFFAOYSA-N

Description

General description

Osmium tetroxide (OsO4) also referred to as osmium, is a heavy tetrahedral, symmetrical molecule. It is both a dye and a non-coagulant fixative agent in electron microscopy primarily used for the examination of biological tissue components in the histopathology laboratory. Specimens are postfixed with osmium tetroxide to ensure the preservation of the lipids.

Application

Osmium tetroxide solution has been used as a fixative in electron microscopy.[1]

Biochem/physiol Actions

Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.

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This Item	75632	201030	419494
Sigma-Aldrich 75633 Osmium tetroxide solution	Sigma-Aldrich 75632 Osmium tetroxide solution	Sigma-Aldrich 201030 Osmium tetroxide	Sigma-Aldrich 419494 Osmium tetroxide
form solution	form solution	form crystalline, powder	form crystalline
Quality Level 100	Quality Level 100	Quality Level 200	Quality Level 200
density 1.0 g/mL at 20 °C	density 1.0 g/mL at 20 °C	density -	density -
concentration 2% in H₂O	concentration 4% in H₂O	concentration -	concentration -
color colorless to yellow	color colorless to yellow	color -	color -
technique(s) electron microscopy: suitable	technique(s) electron microscopy: suitable	technique(s) -	technique(s) -

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pictograms

GHS06,GHS05

signalword

Danger

hcodes

H302 + H332,H310,H315,H318

pcodes

P262 - P280 - P301 + P312 - P302 + P352 + P310 - P304 + P340 + P312 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Storage Class

6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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