09-454

Anti-Dock2 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): dedicator of cyto-kinesis 2, dedicator of cytokinesis 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human, primate, horse
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_004946.2
• UniProt accession no.: Q8C3J5
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... DOCK2(1794)

Description

General description

Dock2 (Dedicator of cytokinesis 2), is a large protein involved in intracellular signaling networks and a member of the DOCK-A subfamily which function as activators of small G proteins. Dock2 specifically activates isoforms of the small G protein Rac and appears to be expressed specifically in leukocytes. These cells express both Rac1 and Rac2 and Dock2 has been shown to bind and promote nucleotide exchange on both of these isoforms. (Nishihara, 1999)

Specificity

09-454 recognizes mouse DOCK2.

Predicted to cross react with human, equine, and primate, based on 93% sequence homology (13/14 aa). The sequence is 85.7% homologous in rat. Reactivity with other species has not been tested.

Immunogen

Epitope: Internal region

KLH-conjugated synthetic peptide near amino acid 1730, within the internal region of mouse DOCK2.

Application

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Western Blotting; Anti-Dock2 Antibody is an antibody against Dock2 for use in WB
Immunohistchemistry (Frozen sections): A representative lot of this antibody dectected DOCK2 in mouse and human brain frozen tissue sections (Cimino, PJ et al. (2009). Am. J. Pathol. 175(4); 1622-1630)

Quality

Evaluated by Western blot on mouse spleen lysate 0.5 μg/mL of Anti-DOCK2 at 1 mg/mL.

Target description

180 kDa

Physical form

Peptide affinity purified

Purified in PBS containing 0.05% NaN3.

Storage and Stability

Maintain at 2-8°C for up to 1 year from date of receipt.

Analysis Note

Control
Mouse spleen lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable