Skip to Content
Merck
All Photos(1)

Documents

U8501

Sigma-Aldrich

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast

Type X, lyophilized powder, ≥40 units/mg protein

Synonym(s):

UTP:α-D-glucose-1-phosphate uridylyltransferase

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9026-22-6
Enzyme Commission number:
2.7.7.9 (BRENDA, IUBMB)
EC Number:
232-811-2
MDL number:
MFCD00132561
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bakers yeast

Quality Level

200

type

Type X

form

lyophilized powder

specific activity

≥40 units/mg protein

composition

Protein, 30-60% modified Warburg-Christian

foreign activity

UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%
inorganic pyrophosphatase ≤0.5%

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) is ubiquitous in plants, yeast, bacteria, and mammals. This enzyme is an octamer of eight identical sub-units.

Application

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast has been used:
  • in the synthesis of uridine-5′-diphosphoglucose (UDP-Glc)-13C9
  • to quantify Suc synthase (SUS) enzyme activity in rice
  • to study the role of hexokinase and glycogen synthase controls the flux in frog oocytes

Biochem/physiol Actions

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) participates in catalyzing the synthesis of UDP-glucose. This enzyme requires divalent cations such as Mg2+, Ca2+, Mn2+, Ni2+ for its activity.

Unit Definition

One unit will form 1.0 μmole of glucose 1-phosphate from uridine-5′-diphosphoglucose and inorganic pyrophosphate per min at pH 7.6 at 25 °C.

Physical form

Lyophilized, sulfate-free powder containing citrate buffer salt

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Starch and Glycogen Biosynthesis
Comprehensive Natural Products II: Chemistry and Biology, 3, 441-495 (1999)
Karina Mariño et al.
Glycobiology, 20(12), 1619-1630 (2010-08-21)
The protozoan parasite Trypanosoma brucei is the causative agent of the cattle disease Nagana and human African sleeping sickness. Glycoproteins play key roles in the parasite's survival and infectivity, and the de novo biosyntheses of the sugar nucleotides UDP-galactose (UDP-Gal)
Qinghua Wang et al.
Plant cell reports, 30(7), 1303-1312 (2011-03-05)
UDP-glucose pyrophosphorylase is an important regulatory enzyme for the development of plants and a critical enzyme in synthesis of glycogen. Here, we reported the cloning of a full-length UGP cDNA from cotton, named GhUGP. Real-time PCR analysis indicated the GhUGP
Lucila I Martínez et al.
Biochimie, 93(2), 260-268 (2010-10-05)
Amoebiasis is an intestinal infection caused by the human pathogen Entamoeba histolytica and representing the third leading cause of death by parasites in the world. Host-parasite interactions mainly involve anchored glycoconjugates localized in the surface of the parasitic cell. In
Activities of enzymes for sucrose-starch conversion in developing endosperm of rice and their association with grain filling in extra-heavy panicle types
Kato T, et al.
Plant Protection Science, 10(4), 442-450 (2007)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service