Skip to Content
Merck
All Photos(1)

Documents

TP0100

Sigma-Aldrich

Total Protein Kit, Micro

Synonym(s):

Bradford

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

description

wavelength 595 nm
total test time 2 min
working reagent stability 18-26°C for 2 weeks
dynamic range 50 mg/dL 0.5 g/L

Quality Level

parameter

18-26 °C temperature
50 μL sample volume

storage temp.

2-8°C

General description

Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.

Application

Total protein kit, micro has been used to determine:
  • total protein in the bacterial homogenate by Bradford method
  • total protein content by the Bradford microassay from the tissue extracts
  • serum total protein
  • soluble protein content by the Bradford method from leaves

Biochem/physiol Actions

Total protein kit, micro can be used to determine the concentration of proteins in solution.

Suitability

Suitable for protein determination for samples of up to 0.5 mg/ml.

Principle

Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.

Testing Method

Colorimetric, Endpoint

Kit Components Also Available Separately

Product No.
Description
SDS

  • Protein Standard 5 mL

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

132.8 °F

Flash Point(C)

56 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Jason G Lieberthal et al.
The Journal of rheumatology, 40(5), 674-683 (2013-04-03)
Glomerulonephritis (GN) is common in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV), but tools for early detection of renal involvement are imperfect. We investigated 4 urinary proteins as markers of active renal AAV: alpha-1 acid glycoprotein (AGP), kidney injury molecule-1 (KIM-1)
Differences in antioxidant mechanisms in grapevines subjected to drought and enhanced UV-B radiation.
Doupis G, et al.
Emirates Journal of Food and Agriculture, 24(6) (2012)
Digital camera-based lipase biosensor for the determination of paraoxon
Pohanka M, et al.
Sensors and Actuators B, Chemical, 273, 610-615 (2018)
Joshua M Halman et al.
Tree physiology, 33(11), 1242-1251 (2013-12-05)
Forests of northeastern North America have been exposed to anthropogenic acidic inputs for decades, resulting in altered cation relations and disruptions to associated physiological processes in multiple tree species, including sugar maple (Acer saccharum Marsh.). In the current study, the
Nagham R El Saidy et al.
Environmental monitoring and assessment, 192(12), 779-779 (2020-11-25)
The remediation of wastewater (WW) is a promising solution for limited water sources. This study aimed to evaluate rice straw (RS) and zeolite (Z) as bioadsorbents for the removal of pollutants, including heavy metals (HMs) (cadmium [Cd], nickel [Ni], and

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service