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SRP6270

Sigma-Aldrich

MMP-2 human

recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)

Synonym(s):

CLG4, CLG4A, MMP-II, MMP2, MONA, TBE-1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

Assay

≥92% (SDS-PAGE)

form

lyophilized powder

mol wt

calculated mol wt 71.8 kDa
observed mol wt 66-71 kDa (DTT-reduced. Ala 30 is the predicted N-terminus.)

packaging

pkg of 10 and 50 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MMP-2(4313)

General description

Matrix metalloproteinase-2 (MMP-2) which is also known as 72kDa type IV collagenase, 72kDa gelatinase, gelatinase A and CLG4A, belongs to the peptidase M10A family. It contains three fibronectin type-II domains and four hemopexin-like domains. MMP-2 is produced by normal skin fibroblasts. The gene encoding this protein is localized on human chromosome 16q12.2.

Biochem/physiol Actions

Matrix metalloproteinase-2 (MMP-2) cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln. It is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. It degrades type IV, V, VII, and X collagens. The protein degrades extracellular matrix proteins and acts on several non-matrix proteins such as big endothelial 1 (bET) and β-type calcitonin gene-related peptide (CGRP) promoting vasoconstriction. MMP-2 also cleaves KISS (metastasis suppressor) at a Gly-|-Leu bond. The protein appears to have a role in myocardial cell death pathways and contributes to myocardial oxidative stress by regulating the activity of glycogen synthase kinase-3 β (GSK3β). It has been shown to cleave GSK3β in vitro. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to fibroblast growth factor-2 (FGF2) and vitronectin.

Physical form

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Disruption of angiogenesis by PEX, a noncatalytic metalloproteinase fragment with integrin binding activity.
Brooks PC,
Cell, 92(3), 391-400 (1998)
Matrix metalloproteinase-2 and myocardial oxidative stress injury: beyond the matrix.
Kandasamy AD
Cardiovascular Research, 85(3), 413-423 (2010)
The association between inflammation-related genes and serum androgen levels in men: the prostate, lung, colorectal, and ovarian study.
Meyer TE
Prostate, 72(1), 65-71 (2012)
Activated interstitial myofibroblasts express catabolic enzymes and mediate matrix remodeling in myxomatous heart valves.
Rabkin E
Circulation, 104(21), 2525-2532 (2001)
Increased expression of 72-kd type IV collagenase (MMP-2) in human aortic atherosclerotic lesions.
Li Z
The American Journal of Pathology, 148(1), 121-128 (1996)

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