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SAB4200382

Sigma-Aldrich

Anti-FAM48A /P38IP antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-C13, Anti-C13ORF19, Anti-FP757, Anti-Family with sequence similarity 48, member A, Anti-SPT20, Anti-bA421P11.4, Anti-p38 interacting protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~95 kDa

species reactivity

mouse, rat, human

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 1-2 μg/mL using monkey COS7 cells
western blot: 1-2 μg/mL using whole extract of human HEK-293T cells
western blot: 2.5-5.0 μg/mL using whole extracts of mouse A20 and rat PC12 cells

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

General description

p38-interacting protein (p38IP), also named family with sequence similarity 48, member A (FAM48A) p38IP comprises nuclear localization sequence, two serine rich domains and a PEST domain. It is localized in the nucleus and the cytoplasm. It partially colocalizes with autophagy related protein 9 (Atg9). p38IP is mapped to human chromosome 13q13.3.

Specificity

Anti-FAM48A /P38IP recognizes human and monkey FAM48A /P38IP.

Immunogen

synthetic peptide corresponding to an internal region of human FAM48A, conjugated to KLH. The corresponding sequence is identical in mouse, rat, bovine, canine and monkey.

Application

Anti-FAM48A /P38IP antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

p38-interacting protein (p38IP) binds directly to p38 mitogen activated protein kinase and is required for its activation in vivo. Both p38 and p38IP were found to be necessary for E-cadherin down-regulation during gastrulation. Recently, p38IP was identified as an interacting protein for autophagy-related protein 9 (mAtg9), a transmembrane protein required for autophagy. The interaction of p38IP with mAtg9 is regulated by p38. p38IP is required for starvation-induced mAtg9 trafficking and autophagosome formation.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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New insights into the function of Atg9
Webber JL and Tooze SA
Febs Letters, 584(7), 1319-1326 (2010)
p38 and a p38-interacting protein are critical for downregulation of E-cadherin during mouse gastrulation
Zohn IE, et al.
Cell, 125(5), 957-969 (2006)
Childhood Apraxia of Speech (CAS) in two patients with 16p11. 2 microdeletion syndrome
Raca G, et al.
European Journal of Human Genetics, 21(4), 455-459 (2013)
Coordinated regulation of autophagy by p38alpha MAPK through mAtg9 and p38IP
Webber JL and Tooze SA
The Embo Journal, 29(1), 27-40 (2010)

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