Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

MDQ1

Sigma-Aldrich

Imprint® Methylated DNA Quantification Kit

To measure global DNA methylation shifts from as low as 10 ng DNA

Synonym(s):

DNA Methylation Quantification, DNA methylation shift

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
41116158
NACRES:
NA.54

General description

Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.

Application

Imprint® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.

Features and Benefits

  • ELISA based format
  • Procedure completes in 4 easy to follow steps
  • All reagents supplied including control methylated DNA
  • Detection limit is 5 ng of fully methylated DNA
  • Input DNA may be as low as 10-200 ng
  • Procedure completes in 3.5 hours
  • 96 well format allows option of studying single samples or high-throughput studies
  • Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
  • May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS
  • M8570Methylated Control DNA, 50 ng/μLSDS

related product

Product No.
Description
Pricing

H9161

Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

H9286

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit, 2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

H7162

Anti-methyl-Histone H3 (Me-Lys9) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

H0913

Anti-acetyl-Histone H3 (Ac-Lys9) antibody, Mouse monoclonal, clone AH3-120, purified from hybridoma cell culture

H2663

Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal, clone HDAC2-62, purified from hybridoma cell culture

R4279

Anti-RbAp46, C-terminal antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

MOD50

Imprint® DNA Modification Kit, For bisulfite DNA conversion & purification

H7789

Anti-acetyl-Histone H1.4 (Ac-Lys26) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

CHP1

Imprint® Chromatin Immunoprecipitation Kit, Complete ChIP reaction in 6 hours in flexible strip well format

SH3011

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Epigenetic Regulators

M8570

Methylated Control DNA, 50 ng/μL

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Warning

Hazard Statements

H290,H317,H412

Hazard Classifications

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Francesca Pirini et al.
Molecular medicine reports, 17(1), 1699-1709 (2017-11-16)
Single nucleotide polymorphisms associated with lipid metabolism and energy balance are implicated in the weight loss response caused by nutritional interventions. Diet‑induced weight loss is also associated with differential global DNA methylation. DNA methylation has been proposed as a predictive
Simone Altmann et al.
Epigenetics, 7(3), 239-252 (2012-03-21)
Recent evidence indicates that maternal nutrition during pregnancy influences gene expression in offspring through epigenetic alterations. In the present study we evaluated the effect of protein excess and deficiency during porcine pregnancy on offspring hepatic and skeletal muscular expression patterns
Felicitas Thol et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 29(18), 2499-2506 (2011-05-18)
To study the incidence and prognostic impact of mutations in Additional sex comb-like 1 (ASXL1) in a large cohort of patients with myelodysplastic syndrome (MDS). Overall, 193 patients with MDS and 65 healthy volunteers were examined for ASXL1 mutations by
Felicitas Thol et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 29(21), 2889-2896 (2011-06-15)
To study the incidence and prognostic impact of mutations in DNA methyltransferase 3A (DNMT3A) in patients with acute myeloid leukemia. A total of 489 patients with AML were examined for mutations in DNMT3A by direct sequencing. The prognostic impact of
Alok Mishra et al.
Methods in molecular biology (Clifton, N.J.), 863, 15-31 (2012-02-24)
Epigenetics is an emerging science that can help to explain carcinogenesis. The possibility that carcinogenesis may originate in a stem cell process was proposed recently. Stem cells are generated and contribute to tumor formation during the process of tumor development.
View All Related Papers

Articles

Global DNA Methylation Quantification

Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.

Global DNA Methylation Quantification

Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.

Global DNA Methylation Quantification

Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.

Global DNA Methylation Quantification

Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.

Protocols

Imprint® Methylated DNA Quantification Kit (MDQ1) Protocol

The Imprint® Methylated DNA Quantification Kit provides a high-throughput, non-radioactive means of measuring global DNA methylation shifts.

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service