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M6395

Sigma-Aldrich

MCDB 105 Medium

With trace elements, L-glutamine and 25mM HEPES, powder, suitable for cell culture

Synonym(s):

Molecular, Cellular, and Development Biology 105 medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: 0.001242 g/L
glucose: 0.72064 g/L (Dextro)
sodium pyruvate: 0.11 g/L
HEPES: 5.958 g/L (25mM)
L-glutamine: 0.3653 g/L

shipped in

ambient

storage temp.

2-8°C

Related Categories

General description

MCDB media has been designed for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP). Each MCDB medium is formulated (qualitatively and quantitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes the growth of a specific cell type. MCDB 105 is derived from the modifications done with MCDB 104 medium. This medium is suitable for the long-term growth of WI-38, MRC-5, and other human diploid fibroblast-like cells.

Application

MCDB 105 Medium has been used to culture:
  • human umbilical vein endothelial cells
  • primary human endometrial stromal cells (ESC)
  • ovarian cancer cell line (OVCAR3)

Quantity

Formulated to contain 14.9 grams of powder per liter of medium.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Reproduction (Cambridge, England), 159(4), 453-463 (2020-01-29)
The differentiation of endometrial stromal cells (ESC), named decidualization, is essential to regulate trophoblast invasion and to support pregnancy establishment and progression. Decidualization follows ESC proliferation and it has been described that cell cycle arrest contributes to a proper decidualization.
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