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L0144

Sigma-Aldrich

Lucifer Yellow CH dipotassium salt

powder

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About This Item

Empirical Formula (Hill Notation):
C13H9K2N5O9S2
CAS Number:
71206-95-6
Molecular Weight:
521.57
Beilstein:
5224942
MDL number:
MFCD00013445
UNSPSC Code:
12171500
PubChem Substance ID:
24896249
NACRES:
NA.77

form

powder

Quality Level

100

color

orange

fluorescence

λex 428 nm; λem 540 nm

storage temp.

room temp

SMILES string

[K+].[K+].NNC(=O)NN1C(=O)c2cc(cc3c(N)c(cc(C1=O)c23)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C13H11N5O9S2.2K/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2

InChI key

HCWYSCVNSCIZCN-UHFFFAOYSA-L

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General description

Lucifer yellow CH is a fixable dye with an excitation and emission spectrum of 430 nm and 540 nm, respectively.LuciferYellow CH (LYCH) is a small, hydrophilic molecule, and highly fluorescent dye that is used for intracellular staining for the studies conducted on various types of cells. It acts as a marker for the detection of well-established tight junctions in smooth muscles, glia, and interstitial cells of Cajal (ICC).

Application

Lucifer yellow CH dipotassium salt has been used:
  • to visualize cell morphology
  • to assess intercellular communication activity through gap junction by scrape-loading assay
  • as a component of the intracellular solution, which filled the dendrites for the visualization of neuronal morphology
  • in the intracellular solution, to intracellularly label recorded neurons

Features and Benefits

Highly fluorescent dye, useful in marking nerve cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Menachem Hanani
Journal of cellular and molecular medicine, 16(1), 22-31 (2011-07-12)
The fluorescent dye Lucifer yellow (LY) was introduced in 1978, and has been extremely useful in studying cell structure and communications. This dye has been used mostly for labelling cells by intracellular injection from microelectrodes. This review describes the numerous
A co-culture model for determining the target specificity of the de novo generated retinal ganglion cells
Teotia P, et al.
Bio-protocol, 7(7) (2017)
Gregor Belušič et al.
Proceedings. Biological sciences, 288(1961), 20211560-20211560 (2021-10-28)
In many butterflies, the ancestral trichromatic insect colour vision, based on UV-, blue- and green-sensitive photoreceptors, is extended with red-sensitive cells. Physiological evidence for red receptors has been missing in nymphalid butterflies, although some species can discriminate red hues well.
The morphology and intrinsic excitability of developing mouse retinal ganglion cells
Qu Juan and Myhr K L
PLoS ONE, 6(7), e21777-e21777 (2011)
In vitro mesenchymal-epithelial transition in NIH3T3 fibroblasts results in onset of low-dose radiation hypersensitivity coupled with attenuated connexin-43 response
Mathur A, et al.
Biochimica et biophysica acta. General subjects, 1862(3), 414-426 (2018)
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