F4512
Anti-Human IgG (whole molecule)−FITC antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Rabbit Anti-Human IgG (whole molecule)−Fluorescein isothiocyanate
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About This Item
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biological source
rabbit
Quality Level
conjugate
FITC conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
storage condition
protect from light
technique(s)
immunofluorescence: 1:64-1:128 using Hep2 cells
storage temp.
−20°C
target post-translational modification
unmodified
General description
IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. FITC (fluorescein isothiocyanate), a fluorochrome dye conjugation is useful in localization of antigen-antibody interaction of biological structures like cells or tissues. Anti-Human IgG (whole molecule) −FITC antibody diluted 1:100 in wash buffer is used in flow cytometry to analyze antibody affinity and virus binding. This antibody can also be used in indirect immunofluorescence antibody test. FITC labeled anti-human IgG antibody reacts specifically with human.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Human IgG (whole molecule)−FITC antibody is specific for human IgG. Rabbit Anti-Human IgG is conjugated to crystalline fluorescein isothiocyanate (FITC) in an alkaline reaction and then further purified to remove free FITC.
Immunogen
IgG from pooled normal human serum
Application
Anti-Human IgG (whole molecule) −FITC antibody may be used as secondary antibody in indirect immunofluorescence and is also used in flow cytometry .
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
The antibody may be used for immunofluorescent staining of human peripheral blood lymphocytes at a working dilution of 1:64. A minimum working dilution of 1:64 was used on acetone fixed mouse liver cells and anti-nuclear antibody positive serum as the primary antibody. For flow cytometry to label NIH3T3 cells, the antibody was used at a working dilution of 1:100. The antibody was also used in ELISA using human sera.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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