E0162

Carboxylesterase 1 isoform c human

recombinant, expressed in baculovirus infected BTI insect cells

• Synonym(s): Carboxylesterase 1 human, carboxylesterase, esterase
• Enzyme Commission number: 3.1.1.1 (BRENDA, IUBMB)
• EC Number: 232-773-7
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in baculovirus infected BTI insect cells
• Quality Level: 200
• form: liquid
• concentration: ≥0.3 mg/mL
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Carboxylesterase 1 (CE1) is a member of a large multigene carboxylesterase α,β-hydrolase family. It is majorly expressed in the liver. CE1 comprises an αβ domain, a central catalytic domain and a regulatory domain.

Application

Carboxylesterase 1 isoform c human has been used as a reference standard in carboxylesterase activity from the mussel for comparison of substrate specificity and inhibition studies. It has also been used as a commercial recombinant protein for the methodological validation of environmental chemical-based inhibition studies.

Biochem/physiol Actions

Carboxylesterase enzymes are responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and heroin. They also hydrolyze long-chain fatty acid esters and thioesters. Carboxylesterase 1 (CE1) catalyzes the formation of cholesteryl esters from cholesterol and fatty acids. Through a transesterification reaction, CE1 also mediates the generation of fatty acid ethyl esters (FAEEs). It also hydrolyzes aromatic and aliphatic esters with preference to small alcohol groups and bulky acyl groups. CE1 metabolizes drug esters and amides carbamates. It participates in the detoxification of environmental toxicants and carcinogens and is useful in pharmacokinetic studies for evaluating pro-drugs.

Physical properties

This product is offered in a volume of 0.5 mL.

Unit Definition

One unit will hydrolyze one nanomole of 4-nitrophenyl acetate per minute at pH 7.4 at 37 °C.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable