DMS 454

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95062832, human lung, Polygonal

• Synonym(s): DMS454 Cells
• UNSPSC Code: 41106514

Properties

• biological source: human lung
• packaging: tube of 5 μg 95062832-DNA-5UG; pkg of vial of cells 95062832-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Polygonal
• products: Calcitonin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian lung, small cell carcinoma

Cell Line Description

The small cell carcinoma DMS 454 was isolated in 1981 from lung tissue of a male patient at time of autopsy. The cells contain dense-core vesicles and secrete calcitonin.

Application

Human lung tumour model, hormone secretion

Culture Medium

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.