A3803
Bovine Serum Albumin
heat shock fraction, lyophilized powder, essentially fatty acid free, ≥98% (agarose gel electrophoresis)
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
About This Item
biological source
bovine
Quality Level
Assay
≥98% (agarose gel electrophoresis)
form
lyophilized powder
mol wt
~66 kDa
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
cell culture | mammalian: suitable
impurities
≤0.005% Fatty acids
loss
≤5.0%, Loss on drying
pH
7
solubility
water: soluble (40 mg/ml)
suitability
suitable for ELISA applications (as diluent)
UniProt accession no.
foreign activity
BT Virus, none detected
VSV Virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
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General description
Application
- to prevent nonspecific adsorption of fibrin matrices to tissue culture plates
- as a constituent in the preparation of albumin droplets
- as a supplement in L-15 medium to increase spermatogonia number without enhancing the growth of somatic cells
- as a standard protein to evaluate metal oxide adsorption isotherms
Among its many applications, BSA is a fatty acid (FA) carrier. Thus when BSA is used in applications like cell culture, it is important to exercise control over particular FA′s for cell culture, because specific fatty acids can affect different cell lines in a variable manner. In order to control fatty acid content in cell culture, fatty acid-free BSA is useful, for researchers to be able fatty acids specific to their cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific FA′s to use in cell culture. The use of FA-free BSA also addresses concerns about endogenous fatty acids that might be potentially present in non-FA-free BSA.
Biochem/physiol Actions
Features and Benefits
- Heat shock fractionated
- Essentially fatty acid-free
Preparation Note
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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