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ROAMYGLL

Roche

Amyloglucosidase

from Aspergillus niger

Synonym(s):

Aspergillus niger, disaccharidase-type a-glucosidase

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About This Item

Enzyme Commission number:
3.2.1.3 (BRENDA, IUBMB)
UNSPSC Code:
12352204

biological source

Aspergillus niger

Quality Level

100

form

lyophilized

specific activity

6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate)
~6 units/mg protein (At 25 °C with glycogen as the substrate.)

mol wt

97 kDa

packaging

pkg of 3,500 U (11202367001)
pkg of 500 U (11202332001)

manufacturer/tradename

Roche

optimum pH

4.6-4.8

storage temp.

2-8°C

General description

Amyloglucosidase, a 1,4-α-D-glucan glucohydrolase, is a disaccharidase–type α-glucosidase. It is synthesized by several Aspergillus genus species. This exo-enzyme is one of the major industrial enzymes. The stability of amyloglucosidase can be increased by immobilization.

Specificity

Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.

Application

Amyloglucosidase from Aspergillus niger is used for the determination of starch.

Biochem/physiol Actions

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4) and α-(1,6) glucosidic bonds of starch and malto-oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate.

The following concentrations should be taken as a guideline:
  • Western blot: 1 to 10 μg/ml


Working solution: Tris-buffered saline containing 0.1% Tween 20.

Storage conditions (working solution): 2 to 8 °C

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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High-potency amyloglucosidase-producing mold of the Aspergillus niger group.
Smiley K L, et al.
Applied Microbiology, 12(5), 455-455 (1964)
Simultaneous amyloglucosidase and exo-polygalacturonase production by Aspergillus niger using solid-state fermentation.
Costa, J et al.
Brazilian Archives of Biology and Technology, 50, 759-766 (2007)
High fructose corn syrup: production, uses and public health concerns.
Parker K, et al.
Biotechnology and Molecular Biology Reviews, 5, 71-78 (2010)
The action of an amyloglucosidase of Aspergillus niger on starch and malto-oligosaccharides.
J H PAZUR et al.
The Journal of biological chemistry, 234(8), 1966-1970 (1959-08-01)
Juliann R Seebauer et al.
Journal of experimental botany, 61(2), 511-519 (2009-11-18)
The relative role of the maternal source and the filial sink in controlling the composition of maize (Zea mays L.) kernels is unclear and may be influenced by the genotype and the N supply. The objective of this study was
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