CBL1304

Anti-Integrin α4 Antibody, clone PS/2

clone PS/2, Chemicon^®, from rat

• Synonym(s): CD49d, VLA-4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: PS/2, monoclonal
• species reactivity: human, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG2bκ
• NCBI accession no.: NM_000885.4
• UniProt accession no.: P13612
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ITGA4(3676) ; mouse ... Itga4(16401)

Description

General description

Virtually all lymphoid and myeloid cells in bone marrow are reactive with the anti-VLA-4 antibody. VLA-4 is absent or minimally detectable on stromal cells. Myeloid cells maintained in Dexter-type long-term cultures of murine bone marrow are positive, as are lymphoid cells from Whitlock-White cultures.

Specificity

Clone PS/2 recognises the murine alpha4 chain of the VLA-4 integrin heterodimer, 150 kDa. The antibody also reacts with human VLA-4.

Reactivity with other species has not been confirmed.

Application

Anti-Integrin α4 Antibody, clone PS/2 detects level of Integrin α4 & has been published & validated for use in FC, IF, IP, IH.

Flow cytometry: 2 μg per 10⁶ cells

Immunohistochemistry on frozen tissue sections

Immunoprecipitation

Cell adhesion assays

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Linkage

Replaces: 04-1131

Physical form

Format: Purified

Purified rat IgG2bκ in 0.01 M phosphate buffer, pH 7.1 containing no preservatives.

Storage and Stability

Maintain at -20ºC for up to 12 months from date of receipt.Upon first use, aliquot into smaller quantities to avoid additional freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable