681667

Wnt Agonist II, SKL2001

The Wnt Agonist II, SKL2001 controls the biological activity of Wnt. This small molecule/inhibitor is primarily used for Biochemicals applications.

• Synonym(s): Wnt Agonist II, SKL2001, 5-(Furan-2-yl)-N-(3-(1H-imidazol-1-yl)propyl)-1,2-oxazole-3-carboxamide, Wnt Pathway Activator VII
• Empirical Formula (Hill Notation): C₁₄H₁₄N₄O₃
• CAS Number: 909089-13-0
• Molecular Weight: 286.29
• MDL number: MFCD14733081
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: pink-red
• solubility: DMSO: 100 mg/mL, clear, red
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: O=C(C1=NOC(C2=CC=CO2)=C1)NCCCN3C=NC=C3

Description

General description

A cell-permeable imidazolyl-isoxazolamide compound that upregulates β-catenin-regulated transcription (CRT; Effective conc. 10 - 40 µM) by blocking/disrupting β-catenin and Axin interaction, thereby preventing β-catenin phosphorylation (Ser³³/Ser³⁷/Thr⁴¹/Ser⁴⁵) and proteasomal degradation, without affecting the activities of GSK-3α/β or 18 other kinases (≤8.5% inhibition by 10 µM SKL2001). Wnt pathway activation by SKL2001 is shown to effectively promote osteoblastogenesis in both human and murine mesenchymal cultures (10 to 40 µM) as well as suppress MDI- (dexamethasone and insulin) stimulated adipogenesis of murine 3T3-L1 preadipocytes (5 to 30 µM).

A cell-permeable imidazolyl-isoxazolamide that upregulates β-catenin-regulated transcription (CRT; 10 - 40 µM) by disrupting β-catenin and Axin interaction, thereby preventing β-catenin phosphorylation (Ser³³/Ser³⁷/Thr⁴¹/Ser⁴⁵) and proteasomal degradation, without affecting the activities of GSK-3α/β or 18 other kinases (≤8.5% inhibition by 10 µM SKL2001). Shown to effectively promote osteoblastogenesis in both human and murine mesenchymal cultures (10 to 40 µM) as well as suppress MDI- (dexamethasone and insulin) stimulated adipogenesis of murine 3T3-L1 preadipocytes (5 to 30 µM).

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Other Notes

Gwak, J., et al. 2012. Cell Res.22, 237.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable