Skip to Content
Merck
All Photos(1)

Key Documents

654250

Sigma-Aldrich

Anti-TNF-α Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-Tumor Necrosis Factor-α

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

human, mouse (weakly)

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

impurities

≤1 EU/mg Endotoxins

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TNF(7124)

General description

Rabbit polyclonal antibody purified by ammonium sulfate precipitation. Recognizes recombinant and monocyte-derived TNF-α.
Recognizes recombinant and monocyte-derived TNF-α. Exhibits some cross-reactivity with lymphotoxin (TNF-β) and mouse TNF-α in L929 cells.
This Anti-TNF-α Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Neutralization Studies, Paraffin Sections, Radioimmunoassay for the detection of TNF-α.

Immunogen

recombinant, human TNF-α

Application

ELISA (see comments)

Frozen Sections (see comments)

Immunoblotting (see comments)

Neutralization Studies (see comments)

Paraffin Sections (see comments)

Radioimmunoassay (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

1 EU corresponds to 0.1 ng endotoxin (EC-6/USP Standard)

Analysis Note

Positive Control
L929 cells

Other Notes

Reacts specifically with natural and recombinant human TNF-α. An approximately 20 µg/ml solution of this antibody has been shown to neutralize 100% of the activity of 0.5 ng/ml recombinant human TNF-α, as measured in the standard L929 cell bioassay. This antibody has also been reported to work for ELISA, frozen sections, immunoblotting, paraffin sections, radioimmunoassay. Endotoxin: ≤5 EU/mg protein as determined by the Biowhittaker Endotect LAL assay. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Josefa P Alameda et al.
Aging, 11(1), 127-159 (2019-01-12)
CYLD is a deubiquitinating enzyme known for its role as a tumor suppressor whose mutation leads to skin appendages tumors and other cancers. In this manuscript we report that the tumor suppressor CYLD, similarly to other renowned tumor suppressor genes

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service