Skip to Content
Merck
All Photos(1)

Documents

2502

Millipore

ReBlot Plus Mild Antibody Stripping Solution, 10x

Synonym(s):

Western blot stripping solution, blot stripping solution

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41116010
eCl@ss:
42029053

form

liquid

packaging

pkg of 50 mL

manufacturer/tradename

Chemicon®
Re-Blot

technique(s)

western blot: suitable

detection method

chemiluminescent

shipped in

wet ice

General description

Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are electro-phoresed on SDS-PAGE and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of Southern and Northern blots, it has been difficult to reuse Western blots.

Stripping and reprobing of Western blots offers several advantages:

1) Conservation of samples that are expensive or available only in limited quantities,

2) Analysis of a given blot using several different antibodies, e.g. subtype- or isoform-specific antibodies,

3) Reanalysis of anomalous results and confirmation with the same or a different antibody,

4) Correcting errors in incubation with the wrong antibody,

5) Cost savings in reagents and time by reusing the same blot.

While antigen and antibody-based immunoaffinity matrices, such as Sepharose conjugates, have been reused many times without compromising antigen-antibody reactivity, the need for pH extremes and chaotropic agents has precluded the application of these methods to Western blotting.

The MILLIPORE Re-Blot Plus Western Blot Mild Antibody Stripping Solution contains specially formulated solutions that quickly and effectively remove antibodies from Western blots without significantly affecting the immobilized proteins.

Advantages of the Re-Blot Plus Western Blot Mild Antibody Stripping Solution include:

  • No pungent smelling β-mercaptoethanol is contained in the Antibody Stripping Solution.
  • Antibody stripping is done at room temperature. No heating of blots is required.
  • Blots can be stripped of antibodies in approximately 15 minutes at room temperature.
  • Blots may be reused in 25 minutes.

Application

The MILLIPORE Re-Blot Plus Western Blot Mild Antibody Stripping Solution is effective for removal of antibodies from Western blots that have been developed with chemiluminescence or radioactive iodine or other isotopes. It is not recommended for stripping colorimetric substrates (TMB, DAB, 4-chloronapthol, etc.), as it is not possible to effectively remove substrates that precipitate at the reaction site.

The Re-Blot Plus Western Blot Mild Antibody Stripping Solution should be used only for qualitative purposes until it has been established by comparative blot analysis that stripping does not quantitatively affect a given antigen.

This product is for research use only; not for diagnostic or in vivo use.

Components

Mild Antibody Stripping Solution (10x) - (2 containers / 25 mL each).

Storage and Stability

The Mild Antibody Stripping Solution should be stored at 2-8°C upon arrival. Product is stable for 3 to 6 months after receipt. If Antibody Stripping Solution crystallizes upon storage, it may be re-dissolved with gentle warming at 37°C before use.

Note: To prevent reagent degradation secure the cap tightly upon storage. Avoid extended exposure to air.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Re-Blot is a trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Storage Class Code

6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Xiaoyu Wang et al.
Proteome science, 9, 53-53 (2011-09-16)
An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity
Cristian Mircean et al.
Bioinformatics (Oxford, England), 21(9), 1935-1942 (2005-01-14)
The protein lysate microarray is a developing proteomic technology for measuring protein expression levels in a large number of biological samples simultaneously. A challenge for accurate quantification is the relatively narrow dynamic range associated with the commonly used chromogenic signal
Nicholas W Baetz et al.
Molecular biology of the cell, 24(5), 643-658 (2013-01-04)
The Rab11-family interacting proteins (Rab11-FIPs) facilitate Rab11-dependent vesicle recycling. We hypothesized that Rab11-FIPs define discrete subdomains and carry out temporally distinct roles within the recycling system. We used live-cell deconvolution microscopy of HeLa cells expressing chimeric fluorescent Rab11-FIPs to examine
Jannike M Andersen et al.
Behavioural brain research, 390, 112676-112676 (2020-05-15)
Activation of calcium/calmodulin-dependent protein kinase II (CaMKII), particularly its α isoform, is known to be important for neuronal processes central for learning and memory and has also been implicated in the maladaptive learning involved in drug addiction.Thr286 autophosphorylation of αCaMKII
C Baldock et al.
The Journal of cell biology, 152(5), 1045-1056 (2001-03-10)
We propose a new model for the alignment of fibrillin molecules within fibrillin microfibrils. Automated electron tomography was used to generate three-dimensional microfibril reconstructions to 18.6-A resolution, which revealed many new organizational details of untensioned microfibrils, including heart-shaped beads from

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service