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Sigma-Aldrich

Anti-β-Tubulin Antibody, clone AA2, Alexa Fluor™ 488 conjugate

clone AA2, Upstate®, from mouse

Synonym(s):

Anti-CDCBM6, Anti-CSCSC1, Anti-M40, Anti-OK/SW-cl.56, Anti-TUBB1, Anti-TUBB5

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AA2, monoclonal

species reactivity

human, rat, bovine, mouse

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

P07437

shipped in

wet ice

target post-translational modification

unmodified

Specificity

β-Tubulin isotypes I, II, III, IVa, and IVb
Predicted to have broad species cross-reactivity based on sequence homology.

Immunogen

Purified bovine brain tubulin; epitope has been mapped to amino acids 412-430

Application

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-β-Tubulin Antibody, clone AA2, Alexa Fluor 488 conjugate is validated for use in FC, IF, WB, IC for the detection of β-Tubulin.

Quality

routinely evaluated by immunoblot on RIPA lysates from 3T3/A31, PC-12, Jurkat, or A431 cells

Target description

50kDa

Physical form

Protein G Purified

Storage and Stability

1 year at 4°C from date of shipment

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Alexa Fluor™ is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rafaela Fernandes Zancan et al.
Scientific reports, 11(1), 4666-4666 (2021-02-27)
In root canal therapy, irrigating solutions are employed to eliminate the bacterial load and also prepare dentin for sealer interaction. The aim of this research was to assess how irrigating solutions employed on their own or in sequence affected the
J M Apgar et al.
Journal of microscopy, 191(Pt 1), 20-27 (1998-09-02)
The fluorescence pattern induced by haematoxylin-eosin (HE) and Masson's trichrome (MT) staining methods on paraffin sections of rat embryos (from 13 to 18 days old) has been studied. Using optimal excitation (green light, 545 nm), HE- or MT-stained sections showed
cAMP, tubulin, axonal transport, and regeneration.
Liu, Howard H and Brady, Scott T
Experimental neurology, 189, 199-203 (2004)
Federico Pellegrini et al.
Cancer investigation, 23(3), 264-273 (2005-06-14)
Anticancer agents that interfere with microtubulin function are in widespread use in man and have a broad spectrum of activity against both hematological malignancies and solid tumors. The mechanisms of actions of these agents have been better defined during the
Crystallographic structure of tubulin: implications for dynamics and drug binding.
Downing, K H and Nogales, E
Cell Structure and Function, 24, 269-275 (1999)
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