06-238
Anti-Gβ Antibody
serum, Upstate®
Synonym(s):
Anti-HG2C1, Anti-NEDHYDF, Anti-SSS4
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
clone
polyclonal
species reactivity
mouse, human
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... GNB2(2783)
Specificity
Gβ subunits; recognizes both isoforms
Immunogen
Internal sequence of G-protein β subunit (KTREGNVRVSREL) conjugated to octavalent polylysine base
Application
Detect Gβ using this Anti-Gβ Antibody validated for use in IP & WB.
Quality
Immunoblot Analysis: A 1:500-1:1000 dilution of this antibody detected the Gβ subunits in 20 μg of a cell lysate prepared from rat brain microsomal preparation (Catalog # 12-144).
Immunoprecipitation/Immunoblot: 10 μl of this antibody immunoprecipitated the Gβ subunit from 500 μg-1mg of a lysate of rat brain microsomal preparation (Catalog # 12-144).
Immunoprecipitation/Immunoblot: 10 μl of this antibody immunoprecipitated the Gβ subunit from 500 μg-1mg of a lysate of rat brain microsomal preparation (Catalog # 12-144).
Target description
35/36kDa
Physical form
PBS, pH 7.2
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
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J Yamauchi et al.
The Journal of biological chemistry, 275(11), 7633-7640 (2000-03-14)
Heterotrimeric G proteins stimulate the activities of two stress-activated protein kinases, c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase in mammalian cells. In this study, we examined whether alpha subunits of G(i) family activate JNK using transient expression system
Importance of the G protein gamma subunit in activating G protein-coupled inward rectifier K(+) channels.
T Kawano, L Chen, S Y Watanabe, J Yamauchi, Y Kaziro, Y Nakajima, S Nakajima, H Itoh
Febs Letters null
J Yamauchi et al.
The Journal of biological chemistry, 274(4), 1957-1965 (1999-01-16)
Heterotrimeric G protein beta gamma subunit (Gbeta gamma) mediates signals to two types of stress-activated protein kinases, c-Jun NH2-terminal kinase (JNK) and p38 mitogen-activated protein kinase, in mammalian cells. To investigate the signaling mechanism whereby Gbeta gamma regulates the activity
J Yamauchi et al.
The Journal of biological chemistry, 272(44), 27771-27777 (1997-11-05)
Various extracellular stimuli activate three classes of mitogen-activated protein kinases (MAPKs): extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 MAPK. In mammalian cells, p38 MAPK is activated by endotoxins, inflammatory cytokines, and environmental stresses. We show here that p38 MAPK
Laura Schultz-Rogers et al.
Molecular genetics & genomic medicine, 8(11), e1477-e1477 (2020-09-13)
GNB1 encodes a subunit of a heterotrimeric G-protein complex that transduces intracellular signaling cascades. Disruptions to the gene have previously been shown to be embryonic lethal in knockout mice and to cause complex neurodevelopmental disorders in humans. To date, the
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