SMB00801

Lipopolysaccharides from Proteus vulgaris

purified by phenol extraction

• Synonym(s): LPS
• UNSPSC Code: 12352211
• NACRES: NA.28

Properties

• biological source: bacterial (Proteus vulgaris)
• Quality Level: 200
• form: lyophilized powder
• purified by: phenol extraction
• impurities: ≤3% Protein (Lowry)
• color: white to yellow cast
• solubility: water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. They consist of lipid A moiety linked to an antigenic O-polysaccharide.
Proteus vulgaris is a rod-shaped Gram-negative, facultative anaerobe bacterium. It inhabits the intestinal tract of humans and animals and can be found in soil, water and feces. P. vulgaris is a member of the Enterobacteriaceae family which are opportunistic pathogens in humans, responsible for urinary tract and burn infections.
The chemical structures of LPS from Proteus sp. are different from each other.

Biochem/physiol Actions

LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular patterns (PAMPs). Different chemical structures of LPS can be associated with the virulence and pathogenesis of the bacteria. It has been reported that the LPS chemical structure from P. vulgaris can influence the crystallization of mineral urine components (such as calcium and magnesium), resulting in stone formation in the kidney. Antibodies produced in rickettsial infections were found to react with LPS from Proteus vulgaris and Proteus mirabilis. This phenomenon allows the purified LPS from these two species to serve as a tool for the diagnosis of rickettsiosis (scrub typhus, caused by the bacterium Orientia sp.) in the Weil-Felix test.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable