ORFBFP
MISSION® TRC3 ORF BFP DNA Control
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About This Item
NACRES:
NA.51
UNSPSC Code:
41106609
Concentration:
500 ng/μL
Pricing and availability is not currently available.
packaging
vial of 20 μL
Quality Segment
concentration
500 ng/μL
application(s)
genome mapping
genomic analysis
shipped in
dry ice
storage temp.
−70°C
General description
The MISSION TRC3 ORF BFP Control Vector is a lentivirus plasmid vector. The vector contains an ORF insert expressing BFP, and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format, including our latest Whole Genome LentiORF Pool.
This positive control can be used for measuring transduction efficiency and optimizing ORF delivery in cell lines that do not survive any puromycin exposure. The BFP Control contains a gene encoding BFP driven by the EF1a promoter. This control provides fast visual confirmation of successful transduction. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively.
In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The MISSION TRC3 ORF BFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
This positive control can be used for measuring transduction efficiency and optimizing ORF delivery in cell lines that do not survive any puromycin exposure. The BFP Control contains a gene encoding BFP driven by the EF1a promoter. This control provides fast visual confirmation of successful transduction. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively.
In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The MISSION TRC3 ORF BFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
Functional Genomics/Overexpression Screening/Target Validation
Legal Information
Use of this product is subject to one or more license agreements. For details, please see the MISSION License Page
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| concentration 500 ng/μL | concentration 500 ng/μL | concentration 1x106 VP/ml (via p24 assay) | concentration 1x106 VP/ml (via p24 assay) |
| storage temp. −70°C | storage temp. −20°C | storage temp. −70°C | storage temp. −70°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| packaging vial of 20 μL | packaging vial of 20 μL | packaging vial of 200 μL | packaging vial of 200 μL |
| application(s) genome mapping | application(s) genome mapping | application(s) genome mapping | application(s) genome mapping |
Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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