N5759

4-Nitrophenyl β-D-cellobioside

chromogenic, ≥98% (TLC), powder

• Synonym(s): p-Nitrophenyl β-D-cellobioside
• Empirical Formula (Hill Notation): C₁₈H₂₅NO₁₃
• CAS Number: 3482-57-3
• Molecular Weight: 463.39
• Beilstein: 100234
• MDL number: MFCD00069845
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897749
• NACRES: NA.32

Properties

• product name: 4-Nitrophenyl β-D-cellobioside, ≥98% (TLC)
• Quality Level: 300
• Assay: ≥98% (TLC)
• form: powder
• solubility: water: 49.00-51.00 mg/mL
• storage temp.: 2-8°C
• SMILES string: OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@@H](O[C@@H]2CO)Oc3ccc(cc3)[N+]([O-])=O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C18H25NO13/c20-5-9-11(22)12(23)14(25)18(30-9)32-16-10(6-21)31-17(15(26)13(16)24)29-8-3-1-7(2-4-8)19(27)28/h1-4,9-18,20-26H,5-6H2/t9-,10-,11-,12+,13-,14-,15-,16-,17-,18+/m1/s1
• InChI key: IAYJZWFYUSNIPN-KFRZSCGFSA-N

Description

Application

4-Nitrophenyl β-D-cellobioside has been used as a substrate to assay β-D-celluliosidase or cellobiohydrolase (exocellulase) activity in the microbial community. It has also been used as a substrate to study the endoglucanase J30 activity.

Biochem/physiol Actions

4-Nitrophenyl β-D-cellobioside is a cellotriose analog, and a chromogenic substrate commonly used for the detection of cellulase activity. Exoglucanase, endoglucanase and β -glucosidase hydrolyze 4-nitrophenyl β-D-cellobioside to form p-nitrophenol (PNP).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves