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Key Documents

MTOX1001

Sigma-Aldrich

MDR1 Knockout Caco-2 Cells

human male colorectal tissue (Source Disease: colon adenocarcinoma)

Synonym(s):

C2BBe1 Cells MDR1 (-/-/-/-)

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

product name

MDR1 Knockout Caco-2 Cells, one vial

biological source

human male colorectal tissue (Source disease: colon adenocarcinoma)

Quality Level

form

liquid

technique(s)

drug transporter assay: suitable
permeability assay: suitable

OMIM accession no.

application(s)

ADME/TOX

storage temp.

−196°C

Gene Information

human ... ABCB1(5243)

General description

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCB1 (MDR1) efflux transporter.

Features and Benefits

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:
  • A functional knockout of the MDR1 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MDR1 substrate
  • The 24 well Transwell format enables the MDR1 knockout cells to be included in standard drug transporter protocols
  • Human assay with no interference from animal inhibitors
  • Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Kit Components Also Available Separately

Product No.
Description
SDS

  • MTOX1001MDR1 Knockout Caco-2 Cells, one vial 1 vialSDS

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Felix Huth et al.
Journal of pharmaceutical sciences, 110(6), 2562-2569 (2021-02-05)
The estimation of the extent of absorption of drug candidates intended for oral drug delivery is an important selection criteria in drug discovery. The use of cell-based transwell assays examining flux across cell-monolayers (e.g., Caco-2 or MDCK cells) usually provide
Angelo E Andres et al.
Methods in molecular biology (Clifton, N.J.), 2430, 449-466 (2022-04-28)
Taxoids such as paclitaxel (Taxol) are an important class of anticancer drugs that bind β-tubulin and stabilize cellular microtubules. To provide new chemical tools for studies of microtubules, we synthesized derivatives of paclitaxel modified at the 7-position with the small
P Artursson
Journal of pharmaceutical sciences, 79(6), 476-482 (1990-06-01)
A human intestinal cell line, Caco-2, was used as a model to study the passive diffusion of drugs across intestinal epithelium. The cells formed continuous monolayers when grown on permeable filters of polycarbonate. After 10 days in culture, the monolayers
Mark I Kaldas et al.
The Journal of pharmacy and pharmacology, 55(3), 307-312 (2003-05-02)
Resveratrol is a dietary constituent suggested to have protective effects against cancer as well as cardiovascular disease. The purpose of the study was to learn whether this agent could be absorbed in man and enter the systemic circulation. This was
S Yee
Pharmaceutical research, 14(6), 763-766 (1997-06-01)
To evaluate and optimize the use of Caco-2 cell monolayers to predict the in vivo absorption of a broad range of compounds in man. Caco-2 cells are derived from human adenocarcinoma colon cells and spontaneously differentiate when grown on porous

Articles

Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.

Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.

Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.

Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.

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