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MAK329

Sigma-Aldrich

L-Lactate Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

L-Lactic Acid Assay Kit, Lactate Quantification Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 colorimetric tests

input

cell
plasma
serum
solution(s) (collagen)

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; cancer

storage temp.

−20°C

General description

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.

Application

The L-Lactate Assay Kit may be used for:
  • Cancer Research
  • Diabetes Research
  • Endocrinological Disease Research

Features and Benefits

Sensitive Detection Range: Detect L-Lactate levels accurately across a wide range, from 0.05 mM to 2 mM, using only 20 μL of sample in a 96-well plate setup, ensuring highly sensitive analysis with minimal sample requirement.

Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 20 minute room temperature reaction, reducing complexity and saving valuable time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Suitability

Suitable for lactate determination in serum, plasma, and cell media samples.

Principle

The Lactate Assay Kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
329CE12A05
329CE09A12
329CE05A14
329CE04A02
329CE09A12

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Katharina Richard et al.
The Journal of experimental medicine, 218(2) (2020-11-21)
Two cosegregating single-nucleotide polymorphisms (SNPs) in human TLR4, an A896G transition at SNP rs4986790 (D299G) and a C1196T transition at SNP rs4986791 (T399I), have been associated with LPS hyporesponsiveness and differential susceptibility to many infectious or inflammatory diseases. However, many
Triiodothyronine prevents tissue hypoxia in experimental sepsis: potential therapeutic implications.
Iordanis S Mourouzis et al.
Intensive care medicine experimental, 9(1), 17-17 (2021-04-10)
Ana Zúñiga et al.
ACS synthetic biology, 10(12), 3527-3536 (2021-12-02)
Bacteria equipped with genetically encoded lactate biosensors are promising tools for biopharmaceutical production, diagnostics, and cellular therapies. However, many applications involve glucose-rich and anoxic environments, in which current whole-cell lactate biosensors show low performance. Here we engineer an optimized, synthetic
Tia R Tidwell et al.
Cancer & metabolism, 10(1), 9-9 (2022-05-17)
Most in vitro cancer cell experiments have been performed using 2D models. However, 3D spheroid cultures are increasingly favored for being more representative of in vivo tumor conditions. To overcome the translational challenges with 2D cell cultures, 3D systems better

Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. For the lactate concentration standard curve, lactate standard solutions have to be prepared. Can these solutions be frozen and used multiple times? This would save a lot of time per Assay.

    1 answer

    1. The L-Lactate standard provided in this kit is prepared as a concentrated solution at 20 mM solution. Following dilution to the recommended working concentration the solution may not be stored. It is best prepared fresh.

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