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L1378

Sigma-Aldrich

L-Lactic Dehydrogenase from bovine muscle

Type X, ammonium sulfate suspension, ≥600 units/mg protein

Synonym(s):

(S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD, Lactate

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bovine muscle

Quality Level

type

Type X

Assay

3.0—25.0 mg protein/mL (biuret)

form

ammonium sulfate suspension

specific activity

≥600 units/mg protein

manufacturer/tradename

Sigma-Aldrich

technique(s)

activity assay: suitable

color

white

pH

7.5

UniProt accession no.

application(s)

life science and biopharma

foreign activity

pyruvate kinase ≤0.01%

storage temp.

2-8°C

Gene Information

General description

Research Area: Cell Signaling
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.

Application

L-Lactic Dehydrogenase from bovine muscle has been used in lactate assay to assess lactate tissue accumulation.

Biochem/physiol Actions

Lactate dehydrogenase (LDH) plays a critical role as a checkpoint in gluconeogenesis and DNA metabolism. It plays a vital role in maintaining homeostasis during periods of low oxygen availability. As an oxidoreductase enzyme, lactate dehydrogenase catalyzes the reversible conversion of pyruvate to lactate using NADH. This enzyme is crucial for the anaerobic metabolism of glucose when oxygen is either absent or in short supply. LDH is the sole serum biomarker effective for evaluating metastatic melanomas.
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physical form

Crystalline suspension in 2.4 M (NH4)2SO4 solution, pH 6.0

Analysis Note

Protein determined by biuret.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Articles

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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