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D2001

Sigma-Aldrich

Deoxyribonucleic acid sodium salt from Escherichia coli strain B

Type VIII

Synonym(s):

DNA

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106305
eCl@ss:
32160414
NACRES:
NA.51

type

Type VIII

Quality Level

form

fibers

impurities

<2% protein

storage temp.

2-8°C

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Application

Escherichia coli DNA has many uses in research. Escherichia coli DNA (ecDNA) is used in studies of DNA binding agents that modulate DNA structure and function. E. coli DNA is used in physicochemical studies of DNA behavior in solution, and to evaluate species-specific DNA structures and binding domains. This DNA is particularly useful for genomic analysis, including PCR, library construction in bacteriophage lambda, and in heterologous pre-hybridization and hybridization protocols.
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used to measure the melting temperature (Tm) and in differential scanning calorimetry (DSC) studies. It has also been used as a template sequence in the polymerase chain reaction (PCR).

Preparation Note

Prepared by procedure of Marmur, J., J. Mol. Biol., 3, 208 (1961).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Differential scanning calorimetry of bacteria
Miles CA, et al.
Microbiology, 132(4), 939-952 (1986)
R Richins et al.
Biotechnology progress, 17(2), 252-257 (2001-04-21)
The Escherichia coli DNA binding protein FIS is a transcriptional modulator involved in the regulation of many cellular processes, including the activation of rRNA synthesis. High-level overproduction of FIS in early, mid, or late log cultures resulted in growth-phase- and
Mitsumasa Hashimoto et al.
The Journal of biological chemistry, 278(31), 28501-28507 (2003-05-16)
Closely opposed lesions form a unique class of DNA damage that is generated by ionizing radiation. Improper repair of closely opposed lesions could lead to the formation of double strand breaks that can result in increased lethality and mutagenesis. In
Solid-state magic-angle spinning NMR of outer-membrane protein G from Escherichia coli
Hiller M, et al.
Chembiochem, 6(9), 1679-1684 (2005)
O Schröder et al.
Biochemical and biophysical research communications, 282(1), 219-227 (2001-03-27)
The DNA-binding protein H-NS compacts DNA and acts as a specific transcription factor regulating the expression of various bacterial genes. The small abundant protein binds to curved DNA without apparent sequence specificity and the exact nature of its DNA interaction

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