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About This Item
NACRES:
NA.81
UNSPSC Code:
41106514
eCl@ss:
32040503
Biological source:
human bronchi
Morphology:
(epithelial)
biological source
human bronchi
product line
PromoCell®
packaging
pkg of 500,000 cells
morphology
(epithelial)
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
General description
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
Our HBEpC are now also available from Air-Liquid Interface pre-screened, HLA-typed, and COPD/Asthma donors.
Our HBEpC are now also available from Air-Liquid Interface pre-screened, HLA-typed, and COPD/Asthma donors.
Application
Primary Human Bronchial Epithelial Cells (HBEpC) are isolated from the surface epithelium of human bronchi and are provided in a cryopreserved format. HBEpC stain positive for cytokeratin. The respiratory epithelia are responsible for the lubrication of the lungs, the maintenance of humidity, and the cleaning of the respiratory tract. They are an important target for drugs, toxins, and carcinogens. In addition, they are involved in many diseases such as cystic fibrosis. Consequently, HBEpC are useful for investigating the function and pathology of the respiratory system.
Biochem/physiol Actions
Bronchi
Preparation Note
Click here for more information.
Analysis Note
Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Other Notes
Recommended Plating Density: 10000 - 15000 cells per cm2Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15
Recommended Primary Cell Culture Media:Link
Legal Information
PromoCell is a registered trademark of PromoCell GmbH
Disclaimer
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
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Storage Class
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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