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B9139

Sigma-Aldrich

Biotin-dextran

mol wt 10,000, Lysine-fixable

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About This Item

MDL number:
UNSPSC Code:
12352201
eCl@ss:
34058011
NACRES:
NA.25

conjugate

biotin conjugate

Quality Level

form

powder

mol wt

10,000

extent of labeling

~2 mol per mol

color

white

solubility

H2O: soluble 50 mg/mL, clear, colorless to faintly yellow

storage temp.

2-8°C

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Application

Biotin-dextran is used in tissue staining to prepare neurons for detection by avidin-conjugated detection systems. It is taken up by neuronal processes and transported bi-directionally. It infiltrates axon boutons in the anterograde direction and dendritic processes in the retrograde direction. Staining can be observed in fixed and sectioned tissue from two days to two weeks after biotin-dextran is injected into the brain or spinal cord. It can also be applied to cut nerve tracts. Biotinylated neurons are detected by either light or electron microscopy following incubation with avidin-horseradish peroxidase conjugate and the electron-dense substrate 3,3′-diaminobenzidine (DAB).

Other Notes

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Victoria L Cressman et al.
The Journal of comparative neurology, 518(14), 2693-2709 (2010-05-28)
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Pflugers Archiv : European journal of physiology, 459(1), 1-9 (2009-07-25)
This study is to determine whether the nucleus of solitary tract (NTS) is an important component of the central neurocircuitry of the cardiac sympathetic afferent reflex (CSAR) and whether the gamma-aminobutyric acid (GABA) in the NTS modulates the CSAR. Renal
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Stroke, 42(1), 186-190 (2010-11-23)
we have shown that anti-Nogo-A immunotherapy to neutralize the neurite growth inhibitory protein Nogo-A results in functional improvement and enhanced plasticity after ischemic stroke in the adult rat. The present study investigated whether functional improvement and neuronal plasticity can be
Karen M Fisher et al.
The Journal of comparative neurology, 526(15), 2373-2387 (2018-07-18)
The corticospinal tract (CST) forms the major descending pathway mediating voluntary hand movements in primates, and originates from ∼nine cortical subdivisions in the macaque. While the terminals of spared motor CST axons are known to sprout locally within the cord

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