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A8604

Sigma-Aldrich

Anti-Annexin V antibody, Mouse monoclonal

clone AN5, purified from hybridoma cell culture

Synonym(s):

Annexin V Antibody, Annexin V Antibody - Monoclonal Anti-Annexin V antibody produced in mouse

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About This Item

MDL number:
MFCD01864074
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AN5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~35 kDa

species reactivity

human

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using HeLa total cell extract

isotype

IgG1

UniProt accession no.

P08758

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ANXA5(308)

General description

Monoclonal Anti-Annexin V (mouse IgG1 isotype) is derived from the hybridoma AN5 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with purified human Annexin V. Annexin V belongs to a class of Ca2+-dependent binding proteins. the amino acid sequence of the annexin V protein consists of a core of four repeats of a highly conserved 70 amino acid residue motif and a unique N-terminal tail. Within each repeat, there is a 17 amino acid residue consensus sequence, which is postulated to form part of the Ca2+ and/or phospholipid binding site.

Immunogen

purified human annexin V.

Application

Monoclonal Anti-Annexin V antibody produced in mouse has been used in:
  • immunocytochemistry
  • indirect immunofluorescence
  • immunohistochemistry
  • western blotting
  • enzyme linked immunosorbent assay (ELISA)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ruyun Du et al.
Journal of proteome research, 9(4), 1805-1821 (2010-02-18)
Toll-like receptor 4 (TLR4) specifically recognizes lipopolysaccharide (LPS) to initiate signal transduction events that modulate host inflammatory responses. Although increasing numbers of genes have been characterized individually for their involvement in TLR4 signaling, the LPS-induced TLR4-mediated signaling pathway and connected
Argyrios Gerasimou et al.
Cellular & molecular biology letters, 14(1), 100-112 (2008-10-08)
Ischemic diseases are characterized by the presence of pro-apoptotic stimuli, which initiate a cascade of processes that lead to cell injury and death. Several molecules and events represent detectable indicators of the different stages of apoptosis. Among these indicators is
Expression of pro-and-anti-apoptotic antigens in the cerebellum of dogs naturally infected with canine distemper virus
Bregano LC, et al.
Brazilian Journal of Veterinary Pathology, 3(2), 16-16 (2010)
Domain IV of Annexin A5 Is Critical for Binding Calcium and Guarantees Its Maximum Binding to the Phosphatidylserine Membrane
Wang J, et al.
Molecules (Basel), 22(12), 2256-2256 (2017)
Wolfram Brune et al.
Journal of virology, 77(21), 11633-11643 (2003-10-15)
Viruses have evolved various strategies to prevent premature apoptosis of infected host cells. Some of the viral genes mediating antiapoptotic functions have been identified by their homology to cellular genes, but others are structurally unrelated to genes of known function.
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