A4605

Anti-iASPP antibody, Mouse monoclonal

clone LXO49.3, purified from hybridoma cell culture

• Synonym(s): Anti-Inhibitory Member of the Apoptosis-stimulating Protein of p53 Family, Anti-RAI, Anti-Rel-associated Inhibitor
• MDL number: MFCD08705587
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: LXO49.3, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~100 kDa
• species reactivity: mouse, human
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 0.1-0.2 μg/mL using total cell extract of human osteogenic sarcoma (U-2-OS)
• isotype: IgG1
• UniProt accession no.: Q8WUF5
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PPP1R13L(10848) ; mouse ... Ppp1r13l(333654)

Description

General description

Monoclonal Anti-iASPP (mouse IgG1 isotype) is derived from the hybridoma LXO49, produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with a recombinant protein encoding residues of human iASPP. The ASPP family of proteins contains three members, ASSP1, ASSP2 and iASPP. iASPP is also known as REL associated inhibitor (RAI). iASPP exists as a 100 kDa form and as a 70 kDa form (iASPP/RAI). It is found both in the nucleus and cytoplasm, while iASPP/RAI is mainly cytosolic. PPP1R13L (protein phosphatase 1 regulatory subunit 13 like) codes for iASPP, that is located on human chromosome 19q13.

iASPP is an inhibitory member of the apoptosis-stimulating protein of p53 (ASPP) family. It is an oncoprotein that associates with Ras, E1A and E7 to transform cells.

Application

Monoclonal Anti-iASPP antibody produced in mouse has been used in:

• immunoblotting
• immunocytochemistry
• immunoprecipitation

Mouse monoclonal Anti-iASPP antibody can be used for western blot (0.1-0.2μg/mL using total cell extract of human osteogenic sarcoma), microarray, immunoprecipitation and immunocytochemistry.

Biochem/physiol Actions

PPP1R13L (protein phosphatase 1 regulatory subunit 13 like)/iASPP inhibits p53-mediated cell death. It interacts and inhibits NFkB p65 RelA. It may act as a therapeutic target for bladder cancer (BC). It modulates cell proliferation and apoptosis. iASPP plays a key role in breast cancer and acute leukemia.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable