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Key Documents

5190

Sigma-Aldrich

CytoSoft®, Discovery Kit

Multiple Elastic Moduli, (0.2, 0.5, 2, 8, 16, 32, 64 kPa)

Synonym(s):

Discovery Kit

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About This Item

EC Number:
UNSPSC Code:
12352202
NACRES:
NA.75

sterility

irradiated

form

solid

packaging

pack of 7 plates

technique(s)

cell culture | mammalian: suitable

capacity

2.0-3.0 mL

size

6 wells

surface area

9.5 cm2

solubility

water: insoluble

General description

The rigidity of the substrate to which cells adhere can have a profound effect on cell morphology and gene expression. CytoSoft® products provide a tool to culture cells on substrates with various rigidities covering a broad physiological range. On the bottom of each well, there is a thin layer of specially formulated biocompatible silicone, whose elastic modulus (rigidity) is carefully measured. The surfaces of the gels in CytoSoft® products are functionalized to form covalent bonds with amines on proteins. This chemical functionalization is stable and the reaction does not require a catalyst, facilitating the coating of the gel surfaces with matrix proteins and plating cells.

The silicone substrates of CytoSoft® products are optically clear and have a low auto-florescence. The layer of silicone in each dish is firmly bonded to the bottom of the dish. Unlike hydrogels (such as polyacrylamide gels), silicone gels are not susceptible to hydrolysis, do not dry nor swell, are resilient and resistant to tearing or cracking, and their elastic moduli (rigidities) remain nearly unchanged during extended storage periods.

CytoSoft® products accommodate the harvesting of cells using enzymes such as trypsin and collagenase. There is no biochemical breakdown of the substrate during or after enzyme treatment, and there are no residuals of the substrate in the sample retrieved from a CytoSoft® plate.

This CytoSoft® product, Catalog Number 5190-7EA, has various elastic moduli of approximately 0.2, 0.5, 2, 8, 16, 32 and 64 kPa in 7 individual 6-well plates. The thickness of the silicone gel is uniform with a ~0.5 mm thick layer of silicone in each dish that is fully compatible with mammalian cell cultures. The plates are packaged individually, sterilized using irradiation and provided with 7 dishes per package.

Legal Information

CytoSoft is a registered trademark of Advanced BioMatrix, Inc.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

CytoSoft® modulus plates analyze substrate stiffness effects on cell behavior across a physiological range for mechanobiology research.

CytoSoft® modulus plates analyze substrate stiffness effects on cell behavior across a physiological range for mechanobiology research.

CytoSoft® modulus plates analyze substrate stiffness effects on cell behavior across a physiological range for mechanobiology research.

CytoSoft® modulus plates analyze substrate stiffness effects on cell behavior across a physiological range for mechanobiology research.

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