ABN51
Anti-Fox-3 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Neuronal nuclei antigen, NeuN antigen
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human, rat
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RBFOX3(146713)
General description
RNA binding protein fox-1 homolog 3 (UniProt: Q8BIF2; also known as Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Fox-3, Neuronal nuclei antigen, NeuN antigen) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID: 52897) in murine species. Fox-3 acts as a pre-mRNA alternative splicing regulator. It regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD). It is widely expressed in brain, including in cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, and in the spinal cord (at protein level). However, Purkinje cells lack Fox-3 expression. It is also found in the retina in the ganglion cells and some cells in the inner nuclear layer, but is absent from the photoreceptor cells and most cells in the inner nuclear layer. In developing mouse it is expressed in the neural tube as early as day E9.5. Fox-3 expression is shown to be up-regulated by retinoic acid in p19 cells during neural differentiation. Knockout mice display significantly reduced brain weight, impaired neurofilament expression and decreased white matter volume, but normal total body mass. Five isoforms of Fox-3 have been described that are generated by alternative splicing.
Specificity
This antibody recognizes Fox-3 at the N-terminus.
Immunogen
Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of mouse Fox-3.
Application
Anti-Fox-3, Cat. No. ABN51, is a highly specific rabbit polyclonal antibody that targets Fox 3/NeuN and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Western Blotting Analysis: 2 µg/mL from a representative lot detected Fox-3 in mouse brain nuclear extract.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Fox-3 in mouse cerebral cortex, mouse hippocampus, and human cerebellum tissues.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Fox-3 in E18 rat cortex cells.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Fox-3 in mouse cerebral cortex, mouse hippocampus, and human cerebellum tissues.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Fox-3 in E18 rat cortex cells.
Quality
Evaluated by Western Blot in mouse e16 brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Fox-3 on 10 µg of mouse e16 brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Fox-3 on 10 µg of mouse e16 brain tissue lysate.
Target description
~46 kDa observed. An uncharacterized band appears at ~68 kDa in some lysates.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse e16 brain tissue lysate
Mouse e16 brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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