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AB5062P

Sigma-Aldrich

Anti-Vesicular GABA Transporter (VGAT) Antibody

Chemicon®, from rabbit

Synonym(s):

Vesicular Inhibitory Amino Acid Transporter

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Pricing and availability is not currently available.

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, monkey, human, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

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This Item
SAB3501172SAB3501170SAB3501171
antibody form

purified antibody (Protein A)

antibody form

purified antibody (Protein A IgG)

antibody form

purified antibody (Protein A)

antibody form

purified antibody (Protein A IgG)

conjugate

unconjugated

conjugate

unconjugated

conjugate

biotin conjugate

conjugate

horse radish peroxidase (HRP) conjugate

species reactivity

human immunodeficiency virus 1

species reactivity

human immunodeficiency virus 1

species reactivity

human immunodeficiency virus 1

species reactivity

human immunodeficiency virus 1

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

monoclonal

clone

monoclonal

clone

monoclonal

clone

monoclonal

General description

Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter (1). VGAT functions in the uptake of GABA and glycine into synaptic vesicles. Since the protein is bound to synaptic vesicles, VGAT antibodies are excellent markers of presynaptic GABAergic and glycinergic neurons.

Specificity

Antibody cross-reactivity with various species is not known. The immunogen sequence is 100% conserved in mouse, human and monkey.
Specific for Vesicular GABA Transporter (VGAT). Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter. The immunogen peptide shows no significant homology with other known proteins.

Immunogen

A 17 amino acid peptide sequence near the carboxy terminal region of rat VGAT (McIntire, S., 1997).

Application

Immunohistochemistry/Immunofluorescence:
2-20 µg/mL of a previous lot was used on paraformaldehyde fixed sections.

ELISA:
1:10,000 – 1:100,000 dilution of a previous lot was used on ELISA, using 50-100 ng of control peptide (Cat. No. AG379) per well.

Western blot:
1-10 µg/mL (Chemiluminescence technique) The expected molecular weight is approximately 55-60 kDa.

Optimal working dilutions must be determined by the end user.
This Anti-Vesicular GABA Transporter (VGAT) Antibody is validated for use in ELISA, IF, IH, WB for the detection of Vesicular GABA Transporter (VGAT).

Quality

Evaluated by Western Blot on Rat retina lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected Vesicular GABA Transporter on 10μg of rat retina lysates.

Target description

57 kDa

Physical form

Purified rabbit polyclonal in buffer containing PBS with 0.1% BSA.

Analysis Note

Control
Brain tissue, rat retina lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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